Takagi T, Kubota H, Oishi S
Institute for Protein Research, Osaka University, Japan.
Electrophoresis. 1991 Jun;12(6):436-8. doi: 10.1002/elps.1150120610.
Molecular sieving effect of crosslinker-free linear polyacrylamide solutions could be visualized in a real-time mode using schlieren optics for electrophoresis of proteins. Since linear polymer solutions are going to find application in capillary electrophoresis (Zhu, M., et al., J. Chromatogr. 1989, 480, 311-319), the above approach may be useful for obtaining basic data of electrophoresis of proteins and nucleic acids in such media under conditions free from the predominant wall effect pertinent to the use of a capillary.
使用纹影光学技术对蛋白质进行电泳,可实时观察无交联剂线性聚丙烯酰胺溶液的分子筛效应。由于线性聚合物溶液将在毛细管电泳中得到应用(朱,M.等人,《色谱杂志》,1989年,480卷,311 - 319页),上述方法可能有助于获取在无毛细管使用时主要壁效应的条件下,蛋白质和核酸在此类介质中电泳的基础数据。