Dampanaboina Lavanya, Yuan Ning, Mendu Venugopal
Department of Plant and Soil Science, Texas Tech University.
Fiber and Biopolymer Research Institute (FBRI), Department of Plant and Soil Science, Texas Tech University.
J Vis Exp. 2021 Jul 24(173). doi: 10.3791/62055.
Lignin is a natural polymer that is the second most abundant polymer on Earth after cellulose. Lignin is mainly deposited in plant secondary cell walls and is an aromatic heteropolymer primarily composed of three monolignols with significant industrial importance. Lignin plays an important role in plant growth and development, protects from biotic and abiotic stresses, and in the quality of animal fodder, the wood, and industrial lignin products. Accurate estimation of lignin content is essential for both fundamental understanding of the lignin biosynthesis and industrial applications of biomass. The thioglycolic acid (TGA) method is a highly reliable method of estimating the total lignin content in the plant biomass. This method estimates the lignin content by forming thioethers with the benzyl alcohol groups of lignin, which are soluble in alkaline conditions and insoluble in acidic conditions. The total lignin content is estimated using a standard curve generated from commercial bamboo lignin.
木质素是一种天然聚合物，是地球上仅次于纤维素的第二丰富的聚合物。木质素主要沉积在植物次生细胞壁中，是一种芳香族杂聚物，主要由三种具有重要工业价值的单木质醇组成。木质素在植物生长发育中起重要作用，可抵御生物和非生物胁迫，并且对动物饲料、木材及工业木质素产品的质量也有影响。准确估计木质素含量对于深入了解木质素生物合成及生物质的工业应用都至关重要。巯基乙酸（TGA）法是一种高度可靠的估算植物生物质中总木质素含量的方法。该方法通过与木质素的苄醇基团形成硫醚来估算木质素含量，硫醚在碱性条件下可溶，在酸性条件下不溶。总木质素含量通过由商业竹木质素生成的标准曲线来估算。
