Song Jae H, Kang Chan, Han Seung Y, Park Won H, Kim Min H, Moon Ji H, Tae Jung Y, Park Hyeon Y, Yoo In H, Park Jong H, Yeo Yong H, Kim Dae Y
Department of Orthopedic Surgery, Konyang University Hospital, Daejeon, Republic of Korea.
Department of Orthopedic Surgery, Regional Rheumatoid and Degenerative Arthritis Center, Chungnam National University Hospital, Daejeon, Republic of Korea.
J Orthop Res. 2022 Jun;40(6):1446-1456. doi: 10.1002/jor.25159. Epub 2021 Aug 21.
There is growing interest in conservative treatment of Achilles tendon rupture. However, the majority of experimental studies of Achilles tendon have been performed by open tenotomy. More appropriate model of conservative treatment of Achilles tendon rupture is required. We performed an experimental study to evaluate whether outcomes differ between open tenotomy and percutaneous tenotomy of the Achilles tendon in rats. The Achilles tendons of 48 rats were transected. The animals were divided into two groups according to surgical technique: open tenotomy or microscopy-assisted percutaneous tenotomy. After 1, 2, and 4 weeks, functional, biomechanical, and histological analyses were performed. Western blot was performed for quantitative molecular analysis at 1 week. The Achilles functional index was superior in the percutaneous tenotomy group, compared with the open tenotomy group, at 1 week. The cross-sectional area was significantly larger in the percutaneous tenotomy group than in the open tenotomy group at 4 weeks. Relative to the native tendons, load to failure and stiffness yielded comparable results at 2 weeks in the percutaneous tenotomy group and at 4 weeks in the open tenotomy group. The histological score was significantly better in the percutaneous tenotomy group than in the open tenotomy group at 1 week. At 1 week, interleukin-1β expression in the open tenotomy group was higher than in the percutaneous tenotomy group. In summary, Achilles tendon healing was substantially affected by the tenotomy method. We presume that our percutaneous tenotomy method might constitute a useful experimental animal model for conservative treatment of Achilles tendon rupture.
目前，对于跟腱断裂的保守治疗越来越受到关注。然而，大多数关于跟腱的实验研究都是通过开放性肌腱切断术进行的。需要一种更合适的跟腱断裂保守治疗模型。我们进行了一项实验研究，以评估大鼠跟腱开放性肌腱切断术和经皮肌腱切断术的结果是否存在差异。将48只大鼠的跟腱切断。根据手术技术将动物分为两组：开放性肌腱切断术组或显微镜辅助经皮肌腱切断术组。在1周、2周和4周后，进行功能、生物力学和组织学分析。在1周时进行蛋白质免疫印迹法以进行定量分子分析。在1周时，经皮肌腱切断术组的跟腱功能指数优于开放性肌腱切断术组。在4周时，经皮肌腱切断术组的横截面积明显大于开放性肌腱切断术组。相对于正常肌腱，经皮肌腱切断术组在2周时和开放性肌腱切断术组在4周时的破坏载荷和刚度产生了相当的结果。在1周时，经皮肌腱切断术组的组织学评分明显优于开放性肌腱切断术组。在1周时，开放性肌腱切断术组中白细胞介素-1β的表达高于经皮肌腱切断术组。总之，肌腱切断术方法对跟腱愈合有显著影响。我们推测，我们的经皮肌腱切断术方法可能构成一种用于跟腱断裂保守治疗的有用实验动物模型。
