Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, Canada.
Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.
Cytoskeleton (Hoboken). 2021 May;78(5):206-213. doi: 10.1002/cm.21684. Epub 2021 Aug 11.
Through the process of chemotactic migration, neutrophils are able to recruit to an inflammatory site and eliminate pathogens, thus playing a vital role in host defense. The process of neutrophil chemotaxis is mediated by dynamic actin reorganization and polymerization. Adseverin, an actin-binding protein and member of the Gelsolin superfamily of proteins, has been hypothesized to regulate goal directed movement through the capping and severing of actin filaments, but has never been investigated in the context of neutrophil chemotaxis. Using an Adseverin knockout mouse model, we show that Adseverin plays a role in subcortical F-actin assembly at the leading edge during chemotaxis through the generation of free barbed ends on existing actin filaments. In addition, in the absence of Adseverin, neutrophils show reduced speed of migration in vitro and in vivo. This study indicates that Adseverin is a regulator of actin filament generation during neutrophil chemotaxis.
通过趋化性迁移，中性粒细胞能够招募到炎症部位并消灭病原体，从而在宿主防御中发挥重要作用。中性粒细胞趋化作用的过程是由动态肌动蛋白重组和聚合介导的。Adseverin 是一种肌动蛋白结合蛋白，也是 Gelsolin 超家族蛋白的成员，它被假设通过肌动蛋白丝的加帽和切断来调节靶向运动，但从未在中性粒细胞趋化作用的背景下进行过研究。使用 Adseverin 敲除小鼠模型，我们表明 Adseverin 通过在现有肌动蛋白丝上生成游离的端帽，在趋化过程中发挥作用，在皮质下 F-actin 组装。此外，在缺乏 Adseverin 的情况下，中性粒细胞在体外和体内的迁移速度降低。这项研究表明，Adseverin 是中性粒细胞趋化过程中肌动蛋白丝生成的调节剂。
