Laboratório de Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
Laboratório de Genética Humana e Médica, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, Pará, Brazil.
Am J Trop Med Hyg. 2021 Aug 9;105(5):1184-1186. doi: 10.4269/ajtmh.21-0296.
Plasmodium malariae infections are often asymptomatic and long-lasting. Mixed infections are often underdetected in areas where P. malariae, P. vivax, and P. falciparum are coendemic. In this study, we described the occurrence of these species circulating as single or mixed infections in Pará state, Brazil, in the Amazon region, with the purpose of clarifying the impact of misidentification of parasite species based only on morphological description using thick blood smear. By using real-time polymerase chain reaction based on the amplification of the mitochondrial DNA, we detected a prevalence of 46% (58/126) mixed infections with 33.3% P. malariae/P. vivax which were read as P. vivax monoinfections by microscopy detection. Our findings confirmed the high circulation of P. malariae in a malaria endemic area in the Brazilian Amazon region.
疟原虫感染通常无症状且持久。在疟原虫、间日疟原虫和恶性疟原虫共同流行的地区，混合感染往往检测不足。在这项研究中，我们描述了这些物种在巴西亚马逊地区帕拉州作为单一或混合感染的发生情况，目的是阐明仅基于厚血涂片的形态描述来识别寄生虫物种时可能出现的错误。通过使用基于线粒体 DNA 扩增的实时聚合酶链反应，我们检测到混合感染的患病率为 46%（58/126），其中 33.3%为疟原虫/间日疟原虫混合感染，而显微镜检测将其读为间日疟原虫单一感染。我们的研究结果证实了恶性疟原虫在巴西亚马逊地区疟疾流行地区的高传播率。
