Branquinho M S, Lagos C B, Rocha R M, Natal D, Barata J M, Cochrane A H, Nardin E, Nussenzweig R S, Kloetzel J K
Superintendência de Controle de Endemias-SUCEN, São Paulo, Brazil.
Trans R Soc Trop Med Hyg. 1993 Jul-Aug;87(4):391-4. doi: 10.1016/0035-9203(93)90008-e.
Anophelines collected indoors and in the peri-domiciliary area in 3 localities in the Amazon region, state of Acre, Brazil, from August 1990 to January 1991 were examined by enzyme-linked immunosorbent assay (ELISA) using specific monoclonal antibodies directed against the repeats of the circumsporozoite proteins of Plasmodium falciparum, P. vivax, P. vivax V247, and P. malariae. Of the 3056 specimens collected, 2610 were Anopheles oswaldoi, 362 A. deaneorum, 60 A. triannulatus and 24 were A. darlingi. The infection rates of A. oswaldoi were 3.41% for P. falciparum, 2.26% for P. vivax, 1.22 for P. vivax VK247, and 0.42% for P. malariae. For A. deaneorum, the infection rates were 2.76% for P. falciparum, 0.55% for P. vivax, and 0.82% for P. vivax VK247. All samples of the other 2 species collected (A. triannulatus and A. darlingi) were negative in the ELISA. There were certain differences in the anopheline distribution and infection rates between these localities, and in one only A. oswaldoi was found to be infected. These results strongly point to A. oswaldoi as the main malaria vector in the region. No difference was found between the potential vectors of P. vivax and P. vivax VK247. The significance of these findings for malaria control is discussed.
1990年8月至1991年1月期间，在巴西阿克里州亚马逊地区的3个地点，对室内和住家周围区域采集的按蚊进行了检测。采用酶联免疫吸附测定（ELISA），使用针对恶性疟原虫、间日疟原虫、间日疟原虫V247株和三日疟原虫环子孢子蛋白重复序列的特异性单克隆抗体。在采集的3056份标本中，2610份为奥斯瓦尔多按蚊，362份为迪氏按蚊，60份为三带按蚊，24份为达林按蚊。奥斯瓦尔多按蚊对恶性疟原虫的感染率为3.41%，对间日疟原虫为2.26%，对间日疟原虫VK247株为1.22%，对三日疟原虫为0.42%。迪氏按蚊对恶性疟原虫的感染率为2.76%，对间日疟原虫为0.55%，对间日疟原虫VK247株为0.82%。采集的其他2种按蚊（三带按蚊和达林按蚊）的所有样本在ELISA检测中均为阴性。这些地点之间按蚊的分布和感染率存在一定差异，其中只有一个地点发现奥斯瓦尔多按蚊受到感染。这些结果有力地表明奥斯瓦尔多按蚊是该地区主要的疟疾传播媒介。间日疟原虫和间日疟原虫VK247株的潜在传播媒介之间未发现差异。讨论了这些发现对疟疾控制的意义。
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