Yuki M
Department of Orthopedic Surgery, Yamagata University School of Medicine, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1987 Oct;61(10):1107-21.
The purpose of this study was to make clear the changes of structural proteins in the ischemic contracture muscle. Ischemia experiments were conducted on the forelimb of canines. Contracture proteins (myosin, actin, alpha-actinin) were isolated and analysed by using SDS-polyacrylamide gel electrophoresis. In the muscle group after 7-hour-ischemia, the muscle structural proteins were changed remarkably, along the time course after the release of tourniquet. After 7 days, myosin heavy chain decreased to 1/2 to 1/3 the original value. It was especially myosin heavy chain that changed remarkably among muscle structural proteins. Actin showed changes after 10-hour-ischemia.
本研究的目的是明确缺血性挛缩肌肉中结构蛋白的变化。对犬的前肢进行缺血实验。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离并分析挛缩蛋白（肌球蛋白、肌动蛋白、α-辅肌动蛋白）。在缺血7小时后的肌肉组中，肌肉结构蛋白在松开止血带后的时间进程中发生了显著变化。7天后，肌球蛋白重链降至原始值的1/2至1/3。在肌肉结构蛋白中，尤其以肌球蛋白重链变化显著。肌动蛋白在缺血10小时后出现变化。
