J. Craig Venter Institute, La Jolla, CA 92137, USA.
J. Craig Venter Institute, Rockville, MD 20850, USA.
Viruses. 2021 Jul 8;13(7):1319. doi: 10.3390/v13071319.
Zika virus (ZIKV) is a mosquito-borne with a positive-sense RNA genome, which are generally transmitted through the bite of an infected mosquito. ZIKV infections could be associated with neurological sequelae that, and otherwise produces similar clinical symptoms as other co-circulating pathogens. Past infection with one member of the genus often induces cross-reactive antibodies against other flaviruses. These attributes complicate the ability to differentially diagnose ZIKV infection from other endemic mosquito-borne viruses, making it both a public health issue as well as a diagnostic challenge. We report the results from serological analyses using arbovirus-specific peptides on 339 samples that were previously collected from 6 countries. Overall, we found that our multiplexed peptide-based ELISA was highly efficient for identifying ZIKV antibodies as early as 2 weeks post infection, and that it correlates with microneutralization, plaque reduction neutralization tests (PRNTs) and commercial tests for ZIKV in previously characterized samples. We observed that seropositivity varied by patient cohort, reflecting the sampling period in relation to the 2015-2016 ZIKV outbreak. This work evaluates the accuracy, specificity, and sensitivity of our peptide-based ELISA method for detecting ZIKV antibodies from geographically diverse regions. These findings can contribute to ongoing serological methods development and can be adapted for use in future studies.
Zika 病毒（ZIKV）是一种通过蚊子传播的正链 RNA 病毒，通常通过感染蚊子的叮咬传播。ZIKV 感染可能与神经后遗症有关，而且会产生与其他流行的病原体相似的临床症状。过去感染过黄病毒属的一种病毒通常会诱导针对其他黄病毒的交叉反应性抗体。这些特征使得难以将 ZIKV 感染与其他地方性蚊媒病毒区分开来，因此它既是一个公共卫生问题，也是一个诊断挑战。我们报告了使用来自 6 个国家的 339 个样本的虫媒病毒特异性肽进行血清学分析的结果。总的来说，我们发现我们的基于多重肽的 ELISA 非常有效地用于识别 ZIKV 抗体，早在感染后 2 周就可以检测到，并且与微中和试验、噬斑减少中和试验（PRNTs）和之前表征样本中的 ZIKV 商业检测相关。我们观察到，血清阳性率因患者群体而异，反映了与 2015-2016 年 ZIKV 爆发相关的采样时间。这项工作评估了我们的基于肽的 ELISA 方法从地理上多样化的地区检测 ZIKV 抗体的准确性、特异性和敏感性。这些发现可以为正在进行的血清学方法开发做出贡献，并可适应未来研究的需要。
