Graduate Program in Science and Technology of Milk and Derivatives, Federal University of Juiz de Fora, Juiz de Fora, Brazil.
Embrapa Dairy Cattle Research Center, Juiz de Fora, Brazil.
J Dairy Res. 2021 Aug;88(3):302-306. doi: 10.1017/S0022029921000595. Epub 2021 Aug 10.
In this Research Communication we evaluate the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify 380 bacteria isolated from cases of bovine mastitis in Brazil. MALDI-TOF MS identifications were compared to previous identifications by biochemical tests and 16S rRNA sequencing. MALDI-TOF MS achieved a typeability of 95.5%. The accuracy of MALDI-TOF MS for the identification of Staphylococcus isolates was 93.2%. The agreement between MALDI-TOF MS and biochemical identification of Streptococcus agalactiae was 96%, however, the agreement between these techniques for identifying other catalase-negative, Gram-positive cocci was lower. Agreement in identifying Gram-negative bacteria at the genus level was 90.5%. Our findings corroborate that MALDI-TOF MS is an accurate, rapid and simple technique for identifying bovine mastitis pathogens. The availability of this methodology in some research institutions would represent a significant step toward increasing the diagnosis and epidemiological studies of bovine mastitis and other animal infectious diseases in Brazil.
在本研究通讯中，我们评估了基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）在鉴定巴西奶牛乳腺炎病例中分离的 380 种细菌中的应用。MALDI-TOF MS 鉴定结果与先前的生化试验和 16S rRNA 测序鉴定结果进行了比较。MALDI-TOF MS 的可分型率为 95.5%。MALDI-TOF MS 鉴定葡萄球菌属的准确率为 93.2%。MALDI-TOF MS 与生化鉴定无乳链球菌的一致性为 96%，然而，这些技术鉴定其他过氧化氢酶阴性革兰氏阳性球菌的一致性较低。在鉴定革兰氏阴性菌属水平上的一致性为 90.5%。我们的研究结果证实，MALDI-TOF MS 是一种准确、快速和简单的技术，可用于鉴定奶牛乳腺炎病原体。在一些研究机构中提供这种方法将代表朝着增加巴西奶牛乳腺炎和其他动物传染病的诊断和流行病学研究迈出的重要一步。
