Myers S L, Brandt K D
Rheumatology Service, Veterans Administration Medical Center, Indianapolis, IN 46202.
J Rheumatol. 1987 Dec;14(6):1150-5.
Osteoarthritis (OA), characterized by cartilage proteoglycan depletion, synovial inflammation and joint effusion, develops after transection of the anterior cruciate ligament of the canine stifle joint. Synovial villi harvested from the unstable knee of dogs 8 to 14 weeks after cruciate ligament transection and studied in organ culture synthesized more 3H-hyaluronic acid/microgram tissue DNA than villi from the contralateral stifle joints (p less than 0.02). Rates of glycosaminoglycan (GAG) synthesis by synovial cells isolated from the OA joints were comparable to those of synovial cells from contralateral joints, but cells from OA joints tended to proliferate more rapidly (p less than 0.01). Synovial conditioned medium prepared with villi from the OA joints stimulated GAG synthesis and proliferation in synovial cell cultures to a greater extent than either medium prepared with villi from the contralateral knees, or unconditioned medium. Pronase digestion eliminated the stimulatory effect of the conditioned medium. Our results suggest that soluble protein(s) released from the synovium may mediate the synovial proliferation and excessive secretion of hyaluronic acid observed in this arthropathy.
骨关节炎（OA）的特征是软骨蛋白聚糖耗竭、滑膜炎症和关节积液，在犬膝关节前交叉韧带横断后发生。在交叉韧带横断后8至14周从犬不稳定膝关节获取的滑膜绒毛，在器官培养中进行研究，其每微克组织DNA合成的3H-透明质酸比来自对侧膝关节的绒毛更多（p<0.02）。从骨关节炎关节分离的滑膜细胞的糖胺聚糖（GAG）合成速率与对侧关节的滑膜细胞相当，但来自骨关节炎关节的细胞增殖更快（p<0.01）。用来自骨关节炎关节的绒毛制备的滑膜条件培养基比用来自对侧膝关节的绒毛制备的培养基或未处理的培养基更能刺激滑膜细胞培养物中的GAG合成和增殖。链霉蛋白酶消化消除了条件培养基的刺激作用。我们的结果表明，从滑膜释放的可溶性蛋白质可能介导了在这种关节病中观察到的滑膜增殖和透明质酸的过度分泌。
