Manicourt D H, Altman R D, Williams J M, Devogelaer J P, Druetz-Van Egeren A, Lenz M E, Pietryla D, Thonar E J
Université de Louvain, International Institute of Cellular and Molecular Pathology, and Saint-Luc University Hospital, Brussels, Belgium.
Arthritis Rheum. 1999 Jun;42(6):1159-67. doi: 10.1002/1529-0131(199906)42:6<1159::AID-ANR12>3.0.CO;2-Q.
To relate the rate of bone resorption to serum levels of both hyaluronan (HA) and antigenic keratan sulfate (KS) in canine experimental osteoarthritis (OA) and to evaluate the effects of calcitonin on these parameters and the OA lesions of the unstable knee.
Twenty-two dogs underwent anterior cruciate ligament transection (ACLT) and 6 dogs underwent sham operation. Urinary pyridinium crosslinks were quantified by high-performance liquid chromatography. Immunoassays quantified hyaluronan (HA) and antigenic KS. Macroscopic and histologic OA lesions were scored. Calcitonin treatment was started on day 14 postsurgery and stopped on either day 49 or day 104 postsurgery. Control dogs and all treated dogs were killed on day 105.
All ACLT joints developed OA. In contrast to sham-operated animals, all operated dogs exhibited an early and sustained rise in the levels of their urinary and serum markers. Calcitonin markedly reduced the levels of these markers and the severity of OA lesions. Furthermore, the longer the period of calcitonin therapy, the lower the score of the OA lesions.
Bone, synovium, and articular cartilage all appear to be involved in the state of hypermetabolism that develops in unstable joints. Furthermore, the rate of bone resorption increases markedly in the early stages of this OA model and is likely to contribute to cartilage breakdown. Since calcitonin reduced the severity of OA changes, this form of therapy may have benefits for humans who have recently experienced a traumatic knee injury.
在犬实验性骨关节炎（OA）中，将骨吸收速率与透明质酸（HA）和抗原性硫酸角质素（KS）的血清水平相关联，并评估降钙素对这些参数以及不稳定膝关节OA病变的影响。
22只犬接受前交叉韧带横断术（ACLT），6只犬接受假手术。通过高效液相色谱法定量尿吡啶交联物。免疫测定法定量透明质酸（HA）和抗原性KS。对宏观和组织学OA病变进行评分。降钙素治疗在术后第14天开始，在术后第49天或第104天停止。对照犬和所有接受治疗的犬在第105天处死。
所有ACLT关节均发展为OA。与假手术动物相比，所有手术犬的尿和血清标志物水平均出现早期且持续的升高。降钙素显著降低了这些标志物的水平以及OA病变的严重程度。此外，降钙素治疗时间越长，OA病变的评分越低。
骨、滑膜和关节软骨似乎均参与了不稳定关节中出现的高代谢状态。此外，在该OA模型的早期阶段，骨吸收速率显著增加，并且可能导致软骨破坏。由于降钙素降低了OA变化的严重程度，这种治疗方式可能对近期遭受膝关节创伤的人类有益。
