Department of Biochemistry, Faculty of Pharmacy, October 6 University, Giza, Egypt.
Curr Pharm Biotechnol. 2022;23(4):609-618. doi: 10.2174/1389201022666210810105804.
Astaxanthin suppressed obesity in rats fed with high-fat diet (HFD) via the restriction of adipose tissue build-out, therefore, improving insulin sensitivity and inflammation. Metformin reduces insulin resistance and may reduce weight.
Investigation of the effects of astaxanthin and metformin in obesity prompted by a high-fat diet.
The present article investigates the effects of astaxanthin and metformin in obesity prompted by a high-fat diet in rats through measuring miRNA222 and 378.
The rats were classified into four classes containing ten albino rats each: Group I (Normal group): nourished with ordinary diet for 8weeks. Group II (Control positive): nourished with a high-fat diet for 8 weeks. Group III: nourished with astaxanthin (50mg/kg)(1/40 LD50) orally plus a high-fat diet for 8weeks. Group IV: nourished with metformin (500mg/kg) orally plus a high-fat diet for 8 weeks.
Leptin, adiponectin, calprotectin and interleukin 6 (IL-6) were assessed by rat-specific ELISA kits. Tumor necrosis factor-alpha (TNF-α), miRNA222 and miRNA378 expressions were quantified by quantitative real-time PCR.
Astaxanthin and metformin have anti-obesity and antioxidant actions and significantly decreased the weight of the body, glucose, insulin, triglycerides, total cholesterol, triglycerides and leptin, as well as plasma calprotectin & IL-6 and increased HDL-C and adiponectin. The liver TNF-α gene expression, adipose tissue miRNA222 and miRNA378 expression were decreased compared to HFD control rats.
Astaxanthin has regulated the aberrant expression of miRNA222 and 378 that may be related to hyperlipidemia and insulin resistance. Accordingly, astaxanthin deserves a clinical trial in the future due to its effects on miRNAs involved in obesity.
虾青素通过限制脂肪组织的生长来抑制高脂肪饮食（HFD）喂养的大鼠肥胖，从而提高胰岛素敏感性和炎症水平。二甲双胍可降低胰岛素抵抗并可能减轻体重。
研究虾青素和二甲双胍对高脂肪饮食诱导肥胖的影响。
本研究通过测量 miRNA222 和 378，探讨虾青素和二甲双胍对高脂肪饮食诱导肥胖大鼠的影响。
将大鼠分为 4 组，每组 10 只白化大鼠：I 组（正常组）：8 周普通饮食喂养。II 组（对照组）：8 周高脂肪饮食喂养。III 组：虾青素（50mg/kg）（1/40 LD50）口服加高脂肪饮食 8 周。IV 组：二甲双胍（500mg/kg）口服加高脂肪饮食 8 周。
用大鼠特异性 ELISA 试剂盒检测瘦素、脂联素、钙卫蛋白和白细胞介素 6（IL-6）。用实时定量 PCR 定量检测肿瘤坏死因子-α（TNF-α）、miRNA222 和 miRNA378 的表达。
虾青素和二甲双胍具有抗肥胖和抗氧化作用，显著降低体重、血糖、胰岛素、甘油三酯、总胆固醇、甘油三酯和瘦素，以及血浆钙卫蛋白和 IL-6，并增加 HDL-C 和脂联素。与 HFD 对照组大鼠相比，肝 TNF-α基因表达、脂肪组织 miRNA222 和 miRNA378 表达降低。
虾青素调节了 miRNA222 和 378 的异常表达，这可能与高脂血症和胰岛素抵抗有关。因此，虾青素由于其对肥胖相关 miRNA 的作用，值得在未来进行临床试验。
