Gilboa Tal, Ogata Alana F, Walt David R
Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Chembiochem. 2022 Jan 5;23(1):e202100358. doi: 10.1002/cbic.202100358. Epub 2021 Sep 12.
Enzymes can be used as biomarkers for a variety of diseases. However, profiling enzyme activity in clinical samples is challenging due to the heterogeneity in enzyme activity, and the low abundance of the target enzyme in biofluids. Single-molecule methods can overcome these challenges by providing information on the distribution of enzyme activities in a sample. Here, we describe the concept of using the single-molecule enzymology (SME) method to analyze enzymatic activity in clinical samples. We present recent work focused on measuring alkaline phosphatase isotypes in serum samples using SME. Future work will involve improving and simplifying this technology, and applying it to other enzymes for diagnostics.
酶可作为多种疾病的生物标志物。然而，由于酶活性的异质性以及生物流体中目标酶的丰度较低，对临床样本中的酶活性进行分析具有挑战性。单分子方法可以通过提供样本中酶活性分布的信息来克服这些挑战。在此，我们描述了使用单分子酶学（SME）方法分析临床样本中酶活性的概念。我们展示了近期利用SME测量血清样本中碱性磷酸酶同工型的工作。未来的工作将包括改进和简化这项技术，并将其应用于其他酶的诊断。
