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通过电子顺磁共振成像观察酶活性：使用碱性磷酸酶顺磁底物的概念和实验。

Imaging of Enzyme Activity by Electron Paramagnetic Resonance: Concept and Experiment Using a Paramagnetic Substrate of Alkaline Phosphatase.

机构信息

In vivo Multifunctional Magnetic Resonance (IMMR) Center, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, West, Virginia, 26506, USA.

Department of Biochemistry, West Virginia University, School of Medicine, Morgantown, WV, 26506, USA.

出版信息

Angew Chem Int Ed Engl. 2018 Sep 3;57(36):11701-11705. doi: 10.1002/anie.201806851. Epub 2018 Aug 7.

DOI:10.1002/anie.201806851
PMID:30003653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6327950/
Abstract

Enzyme activities are well established biomarkers of many pathologies. Imaging enzyme activity directly in vivo may help to gain insight into the pathogenesis of various diseases but remains extremely challenging. In this communication, we report the use of EPR imaging (EPRI) in combination with a specially designed paramagnetic enzymatic substrate to map alkaline phosphatase activity with a high selectivity, thereby demonstrating the potential of EPRI to map enzyme activity.

摘要

酶活性是许多病理的明确生物标志物。直接在体内对酶活性进行成像可能有助于深入了解各种疾病的发病机制，但仍然极具挑战性。在本通讯中，我们报告了使用电子顺磁共振成像（EPRI）结合专门设计的顺磁酶底物来高选择性地绘制碱性磷酸酶活性图，从而证明了 EPRI 绘制酶活性图的潜力。

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