Department of Microbiology, School of Medicine, Kyungpook National University, Jung-gu, Daegu, South Korea.
Department of Microbiology, Chonnam National University Medical School, Gwangju South Korea.
Virulence. 2021 Dec;12(1):2122-2132. doi: 10.1080/21505594.2021.1961660.
, a major nosocomial pathogen, survives in diverse hospital environments, and its multidrug resistance is a major concern. The ppGpp-dependent stringent response mediates the reprogramming of genes with diverse functions in several bacteria. We investigated whether ppGpp is involved in pathogenesis by examining biofilm formation, surface motility, adhesion, invasion, and mouse infection studies. Transcriptome analysis of early stationary phase cultures revealed 498 differentially-expressed genes (≥ 2-fold change) in a ppGpp-deficient strain; 220 and 278 genes were up and downregulated, respectively. C operon expression, important in pilus biosynthesis during early biofilm formation, was significantly reduced in the ppGpp-deficient strain. Our findings suggest that ppGpp signaling influences biofilm formation, surface motility, adherence, and virulence. We showed the association between ppGpp and pathogenicity in for the first time; ppGpp may be a novel antivirulence target in
, 一种主要的医院病原体，能在各种医院环境中存活，其多药耐药性是一个主要关注点。ppGpp 依赖性严格反应介导了几种细菌中具有不同功能的基因的重编程。我们通过研究生物膜形成、表面运动、黏附、侵袭和小鼠感染研究，来研究 ppGpp 是否参与了 的发病机制。早期静止期培养物的转录组分析显示，在 ppGpp 缺陷型 菌株中，有 498 个差异表达基因（≥ 2 倍变化）；分别有 220 个和 278 个基因上调和下调。在早期生物膜形成过程中对菌毛生物合成很重要的 C 操纵子表达在 ppGpp 缺陷型菌株中显著降低。我们的研究结果表明，ppGpp 信号影响 的生物膜形成、表面运动、黏附和毒力。我们首次证明了 ppGpp 与 中的致病性之间存在关联；ppGpp 可能是 中的一种新型抗毒力靶标。
