Centre de Recherche Scientifique et Techniques sur les Régions Aride (CRSTRA), Biskra, Algeria; Laboratoire de Recherche sur les Zones Arides (LRZA), Université des Sciences et de la Technologie Houari Boumediene (USTHB), BP32 El-Alia, 16111 Bab Ezzouar, Alger, Algeria.
Laboratoire de Recherche sur les Zones Arides (LRZA), Université des Sciences et de la Technologie Houari Boumediene (USTHB), BP32 El-Alia, 16111 Bab Ezzouar, Alger, Algeria.
J Ethnopharmacol. 2021 Dec 5;281:114494. doi: 10.1016/j.jep.2021.114494. Epub 2021 Aug 8.
Marrubium deserti De Noé (Lamiaceae) commonly known as desert horehound, is a small perennial herb distributed in arid climates. This plant has been used in Algeria for the treatment of cold, cough, fever and inflammation.
This study aimed to perform a phytochemical analysis and to evaluate the effect of diethyl ether (ExDiEth), n-butanol (ExBut) and hydroalcoholic (ExHyD) extracts of the aerial parts of M. deserti De Noé on inflammation, nociceptive response and fever in animal models.
The total phenolic content and the different phenolic fraction (flavones-flavonols, free aglycones, anthocyanidins, C-glycosides and flavonic heterosides) were quantified in order to study the chemical diversity. The anti-inflammatory effect was evaluated using carrageenan-induced paw oedema test and the nociceptive response was determined by the chemical method using acetic acid-induced writhing response and formalin-induced paw licking tests and by the thermal method using the hot plate in mice. In addition, anti-fever was investigated using yeast-induced pyrexia in rats.
Quantitative analysis revealed that among all extracts, ExBut had a higher phenolic content and the aerial parts of M. deserti De Noé collected from two different sites (Biskra and Tamanresset) had a significant difference in the contents of flavonic heterosids and flavones-flavonols. PCA and CA showed a strong intra-specific variability of M. deserti De Noé individuals. Our results showed that the ExDiEth, ExBut and ExHyD treatments prevented the increase of paw oedema by 56.25%, 66.65% and 45.56% inhibition percent, respectively. Moreover, treatments with these extracts significantly (p<0.05) decreased the number of writhing and increased the reaction time to thermal stimulation as well as demonstrated reduction in paw licking time both at early and late phases. Likewise, they also showed a significant reduction of yeast-induced pyrexia till 4 h of treatment.
Results from this study show that M. deserti De Noé aerial parts exhibited anti-inflammatory, analgesic with central and peripheral actions and antipyretic activities which could be associated with the presence of some phytochemicals as flavones, flavonols, flavonic heterosids and phenolic acids.
Marrubium deserti De Noé（唇形科）俗称沙漠苦薄荷，是一种分布在干旱气候中的小型多年生草本植物。这种植物在阿尔及利亚被用于治疗感冒、咳嗽、发烧和炎症。
本研究旨在对 M. deserti De Noé 的地上部分的乙醚（ExDiEth）、正丁醇（ExBut）和水醇（ExHyD）提取物进行植物化学分析，并评估其对炎症、疼痛反应和发热动物模型的影响。
为了研究化学成分的多样性，对总酚含量和不同酚类成分（黄酮-黄酮醇、游离糖苷、花青素、C-糖苷和黄酮杂糖苷）进行了定量分析。采用角叉菜胶诱导的足肿胀试验评估抗炎作用，采用醋酸诱导的扭体反应和福尔马林诱导的舔足试验以及热板试验评估疼痛反应，采用酵母诱导的发热试验评估解热作用。
定量分析表明，在所有提取物中，ExBut 的酚类含量最高，而采自两个不同地点（Biskra 和 Tamanresset）的 M. deserti De Noé 地上部分在黄酮杂糖苷和黄酮-黄酮醇的含量上存在显著差异。PCA 和 CA 显示 M. deserti De Noé 个体具有很强的种内变异性。结果表明，ExDiEth、ExBut 和 ExHyD 处理分别抑制了 56.25%、66.65%和 45.56%的足肿胀增加。此外，这些提取物处理显著（p<0.05）减少了扭体次数，增加了对热刺激的反应时间，同时减少了早期和晚期的舔足时间。同样，它们还显著降低了酵母诱导的发热，直到治疗 4 小时。
本研究结果表明，M. deserti De Noé 地上部分具有抗炎、镇痛（中枢和外周作用）和退热活性，这可能与某些植物化学物质的存在有关，如黄酮类、黄酮醇类、黄酮杂糖苷类和酚酸类。
