Laboratory of Phytotherapy Applied to Chronic Diseases, Faculty of Natural and Life Sciences, University Ferhat Abbas Setif 1, Setif, 19000, Algeria.
CiTCoM UMR 8038 CNRS, Health Faculty, UFR Pharmacy, University of Paris, Paris, France.
J Ethnopharmacol. 2021 May 10;271:113881. doi: 10.1016/j.jep.2021.113881. Epub 2021 Jan 27.
Erica arborea known as Khlenj in Algeria is a small shrub belonging to Ericaceae family. E. arborea Aqueous extract (EAAE) is used in traditional medicine for anti-inflammatory, diuretic, antimicrobial, and antiulcer purposes.
To our knowledge, no data reveal the combination between in-vivo anti-inflammatory and toxicological studies of EAAE. For this purpose, the aim of this study is to evaluate the biological activity cited above and assess its safety.
Anti-inflammatory activity was undergone using carrageenan-induced paw edema and croton oil-induced ear edema. The acute and sub-acute toxicity were conducted following the OECD guidelines 423 and 407, respectively. Phytochemical identification was carried out using HPLC-DAD-MS. Quantitative evaluation of polyphenols; flavonoids and antioxidant activity of EAAE were also determined.
Oral administration of EAAE (250 and 500 mg/kg) significantly (p < 0.05) reduced the edema induced by carrageenan. Administration of EAAE dosed at 250 and 500 mg/kg exhibited efficacy in reducing edema induced by croton oil. The acute administration of EAAE at doses of 2000 and 5000 mg/kg did not cause any mortality or adverse effects indicating that the LD is above 5000 mg/kg. The prolonged administration of EAAE (500 and 1000 mg/kg) showed a significant reduction in triglycerides levels in male and female rats whereas no significant changes in other biochemical and hematological parameters were observed. Histopathological damages were recorded in both liver and kidney animal's tissues of both sexes treated with medium and maximum doses of EAAE. Phytochemical characterization of EAAE revealed a high amount of phenolic compounds, HPLC-DAD-MS analysis led to the identification of chlorogenic acid and five flavonol glycosides: myricetin pentoside, quercetin-3-O-glucoside, myricetin-3-O-rhamnoside, quercetin-3-O-pentoside, and quercetin-3-O-rhamnoside.
In the light of the results obtained in this study, EAAE corroborates the popular use to treat the anti-inflammatory impairments. EAAE can be considered as non-toxic in acute administration and exhibited a moderate toxicity in sub-acute administration. High phenolic content and in-vitro antioxidant activity observed indicate that EAAE may reduce oxidative stress markers in-vivo.
在阿尔及利亚，被称为 Khlenj 的 Erica arborea 是一种属于 Ericaceae 科的小灌木。E. arborea 水提取物 (EAAE) 在传统医学中用于抗炎、利尿、抗菌和抗溃疡。
据我们所知，没有数据显示 EAAE 的体内抗炎和毒理学研究相结合。为此，本研究的目的是评估上述生物活性并评估其安全性。
采用角叉菜胶诱导的足肿胀和巴豆油诱导的耳肿胀来进行抗炎活性研究。急性和亚急性毒性分别按照 OECD 指南 423 和 407 进行。使用 HPLC-DAD-MS 进行植物化学鉴定。还测定了 EAAE 中多酚、类黄酮的定量评价和抗氧化活性。
口服 EAAE（250 和 500mg/kg）可显著（p<0.05）减轻角叉菜胶诱导的水肿。EAAE 剂量为 250 和 500mg/kg 时，对巴豆油诱导的水肿有疗效。EAAE 的急性给药剂量为 2000 和 5000mg/kg 不会引起任何死亡率或不良反应，表明 LD 大于 5000mg/kg。EAAE 的长期给药（500 和 1000mg/kg）可显著降低雄性和雌性大鼠的甘油三酯水平，而其他生化和血液学参数没有明显变化。用 EAAE 中剂量和最大剂量治疗的两性动物的肝和肾组织中均记录到组织病理学损伤。EAAE 的植物化学特征显示出高含量的酚类化合物，HPLC-DAD-MS 分析导致鉴定出绿原酸和五种黄酮醇糖苷：杨梅素五糖苷、槲皮素-3-O-葡萄糖苷、杨梅素-3-O-鼠李糖苷、槲皮素-3-O-戊糖苷和槲皮素-3-O-鼠李糖苷。
根据本研究的结果，EAAE 证实了其在治疗抗炎损伤方面的民间用途。EAAE 在急性给药时无毒性，在亚急性给药时表现出中等毒性。观察到高酚含量和体外抗氧化活性表明，EAAE 可能会降低体内氧化应激标志物。
