Division of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Division of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Biochem Biophys Res Commun. 2021 Oct 8;573:13-18. doi: 10.1016/j.bbrc.2021.08.003. Epub 2021 Aug 3.
To assess the effects of a ketogenic diet on metabolism and renal fibrosis in spontaneously hypertensive rats.
Male spontaneously hypertensive rats (SHRs) and Wistar Kyoto (WKY) rats were randomly divided into a ketogenic diet group and a normal diet group. Blood glucose and metabolites were measured after 4 weeks. Renal autophagy-related protein expression was detected by Western blot, and renal fibrosis was detected by Masson staining.
Compared with the normal diet, the ketogenic diet led to significantly decreased glucose tolerance and metabolism; overactivated the renin-angiotensin-aldosterone system; and reduced renal autophagy-related protein expression in SHRs; Masson staining and other experiments showed that the ketogenic diet had no significant effect on hypertensive renal fibrosis.
A Ketogenic diet could lead to disorders of glucose and lipid metabolism, increase hypertension by activating the RAAS, reduce renal autophagy levels and aggravate renal parenchymal damage. Therefore, a ketogenic diet, as a kind of natural therapy, should be vigilantly monitored to prevent further damage in patients with hypertension.
评估生酮饮食对自发性高血压大鼠代谢和肾纤维化的影响。
雄性自发性高血压大鼠（SHRs）和 Wistar Kyoto（WKY）大鼠被随机分为生酮饮食组和正常饮食组。4 周后测量血糖和代谢物。通过 Western blot 检测肾脏自噬相关蛋白的表达，通过 Masson 染色检测肾纤维化。
与正常饮食相比，生酮饮食导致葡萄糖耐量和代谢明显下降；肾素-血管紧张素-醛固酮系统过度激活；并且降低了 SHR 中与肾脏自噬相关的蛋白表达；Masson 染色等实验表明，生酮饮食对高血压性肾纤维化没有明显影响。
生酮饮食可导致糖、脂代谢紊乱，通过激活 RAAS 增加高血压，降低肾脏自噬水平并加重肾实质损伤。因此，生酮饮食作为一种天然疗法，应密切监测，以防高血压患者的病情进一步恶化。
