鞘翅目特异的 StaufenC 具有类似 Loquacious-PD 的功能，参与 dsRNA 加工。
Coleopteran-specific StaufenC functions like Loquacious-PD in dsRNA processing.
机构信息
Department of Entomology, College of Agriculture, Food and Environment, University of Kentucky, Kentucky, USA.
Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju, Republic of Korea.
出版信息
RNA Biol. 2021 Oct 15;18(sup1):467-477. doi: 10.1080/15476286.2021.1960687. Epub 2021 Aug 11.
In , PD isoform of the double-stranded RNA binding protein (dsRBP) Loquacious (Loqs-PD) facilitates dsRNA cleavage to siRNA by Dicer-2. StaufenC (StauC) was discovered as a coleopteran-specific dsRBP required for dsRNA processing in coleopteran insects. Here, we show that StauC is essential for the high RNAi efficiency observed in coleopterans. Knockdown of StauC but not the homologs of Loqs-PD and R2D2 evoked a long-lasting insensitivity to RNAi in the coleopteran cell line, Ledp-SL1. The dsRNA insensitivity induced by StauC knockdown could not be overcome merely by an increase in dose or time of exposure to dsRNA or expression of Loquacious or R2D2. Furthermore, StauC but not Loqs and R2D2 are required for processing of dsRNA into siRNA. StauC overexpression also partly restored the impaired RNAi caused by the knockdown of Loqs-PD in Kc cells. However, StauC was unable to compensate for the loss-of-the function of Dcr-2 or R2D2. Overall, these data suggest that StauC functions like Lops-PD in processing dsRNA to siRNA.
在 中，双链 RNA 结合蛋白（dsRBP）loquacious（Loqs-PD）的 PD 同工型通过 Dicer-2 促进 dsRNA 切割为 siRNA。StaufenC（StauC）被发现是一种鞘翅目特异性 dsRBP，是鞘翅目昆虫中 dsRNA 加工所必需的。在这里，我们表明 StauC 是鞘翅目昆虫中观察到的高 RNAi 效率所必需的。敲低 StauC 但不敲低 Loqs-PD 和 R2D2 的同源物，会在鞘翅目细胞系 Ledp-SL1 中引起持久的对 RNAi 的不敏感性。由 StauC 敲低引起的 dsRNA 不敏感性不能仅仅通过增加 dsRNA 的剂量或暴露时间或表达 Loquacious 或 R2D2 来克服。此外，StauC 而不是 Loqs 和 R2D2 是将 dsRNA 加工成 siRNA 所必需的。StauC 的过表达也部分恢复了在 Kc 细胞中敲低 Loqs-PD 引起的 RNAi 受损。然而，StauC 无法弥补 Dcr-2 或 R2D2 功能的丧失。总的来说，这些数据表明 StauC 像 Loqs-PD 一样在将 dsRNA 加工成 siRNA 中发挥作用。
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