El-Sharkawy Nabela I, Abd-Elhakim Yasmina M, Alklech Alklech M
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Forensic Sci Res. 2019 Jan 31;6(2):159-167. doi: 10.1080/20961790.2018.1541537. eCollection 2021.
There is a dearth of information on the mutual interaction between metal intoxication and adipocere formation. Herein, 40 adult female albino rats were distributed into two equal groups, one used as control while the other orally administered single dose of cadmium chloride (CdCl) 225 mg/kg·bw (LD). Control group was killed by cervical dislocation. Half of dead rats of both groups were subjected for determination of iodine value and estimation of cadmium (Cd) residues while the other half of both groups were submerged in opened glass container previously filled with 4 L dechlorinated tap water kept in closed room with an open air access (one cadaver/container). Gross morphological changes of submerged cadavers were recorded weekly along the experiment. At the end of the experiment, after 3 months, samples were collected again for iodine value determination and estimation of Cd residues. The obtained results revealed the depressant effect of Cd toxicity on development of adipocere. Cd residues were found in different tissues of cadavers at time of death with the highest amount in the intestines followed by the liver and kidneys, then lungs, adipose tissue, muscles, and finally the bones. After 3 months of water submersion, tissues exhibited significant decrease in the amount of Cd residues but to a limit that was still detected. This study concluded the possibility of detection of Cd residues even after adipocere formation. Additionally, it shed light on the possibility of the interference of environmental pollution with the natural rate of decomposition especially adipocere formation.
关于金属中毒与尸蜡形成之间的相互作用，目前缺乏相关信息。在此，将40只成年雌性白化大鼠平均分为两组，一组作为对照组，另一组经口给予单剂量225毫克/千克体重的氯化镉（CdCl）（半数致死剂量）。对照组通过颈椎脱臼处死。两组死亡大鼠的一半用于测定碘值和估算镉（Cd）残留量，另一半则浸没在预先装有4升脱氯自来水的开口玻璃容器中，容器放置在有空气流通的封闭房间内（一个尸体/容器）。在实验过程中，每周记录浸没尸体的大体形态变化。实验结束时，即3个月后，再次采集样本进行碘值测定和Cd残留量估算。所得结果显示，镉毒性对尸蜡形成有抑制作用。在死亡时，尸体的不同组织中均检测到Cd残留，其中肠道中含量最高，其次是肝脏和肾脏，然后是肺、脂肪组织、肌肉，最后是骨骼。水浸3个月后，组织中的Cd残留量显著下降，但仍可检测到。本研究得出结论，即使在尸蜡形成后仍有可能检测到Cd残留。此外，该研究还揭示了环境污染可能干扰自然分解速率，尤其是尸蜡形成的可能性。
