Muckey Mary B, Jones Cassandra K, Woodworth Jason C, Paulk Chad B, Dritz Steve S, Gebhardt Jordan T
Department of Grain Science and Industry, College of Agriculture, Kansas State University, Manhattan KS 66506, USA.
Department of Animal Sciences and Industry, College of Agriculture, Kansas State University, Manhattan KS 66506, USA.
Transl Anim Sci. 2021 Jul 19;5(3):txab121. doi: 10.1093/tas/txab121. eCollection 2021 Jul.
Porcine epidemic diarrhea virus (PEDV) is a possible biological hazard in feed mills. If the virus enters a feed mill, it becomes widely distributed and is difficult to decontaminate from both feed contact and non-feed contact surfaces. The objective of this study was to evaluate a variety of liquid and dry decontamination treatments that could be used to reduce the amount of PEDV found on feed manufacturing surfaces. This experiment was designed as a 5 × 10 factorial with five different feed manufacturing surfaces and 10 decontamination treatments with three replicates of each combination. Surfaces included stainless steel, solid polyethylene, woven polypropylene tote bag, rubber, and sealed concrete coupons. One mL (1×10 TCID/mL) of stock PEDV was applied to each surface and allowed to dry completely for 60 min. Next, for decontamination requiring surface application, the application was performed and allowed 15 min contact time. The quantity of PEDV RNA was determined using quantitative reverse transcription PCR. A decontamination treatment × surface interaction was observed ( < 0.0001), indicating the efficacy of treatment is dependent upon the surface in which it is applied. Within the cement surfaces, the sodium hypochlorite resulted in the greatest ( < 0.05) cycle threshold value, followed by formaldehyde which had a greater ( < 0.05) Ct value compared to remaining treatments. Within polyethylene, rubber, and stainless steel surfaces, the formaldehyde treated surfaces had the greatest Ct values ( < 0.05), followed by the sodium hypochlorite treatment, with other treatments all having lower Ct values ( < 0.05). For the woven polyethylene surfaces, the formaldehyde and sodium hypochlorite treatments had greater Ct values compared to all other treatments ( < 0.05). Additional research is necessary to identify the role of decontamination treatment on PEDV infectivity and develop methods for decontamination of feed manufacturing facilities.
猪流行性腹泻病毒（PEDV）在饲料厂中可能构成生物危害。如果该病毒进入饲料厂，它会广泛传播，并且难以从饲料接触表面和非饲料接触表面清除。本研究的目的是评估各种液体和干式去污处理方法，这些方法可用于减少饲料生产表面上发现的PEDV数量。本实验设计为5×10析因实验，有五种不同的饲料生产表面和10种去污处理方法，每种组合重复三次。表面包括不锈钢、实心聚乙烯、编织聚丙烯手提袋、橡胶和密封混凝土试块。将1 mL（1×10 TCID/mL）的PEDV原液应用于每个表面，并使其完全干燥60分钟。接下来，对于需要表面应用的去污处理，进行处理并给予15分钟的接触时间。使用定量逆转录PCR测定PEDV RNA的数量。观察到去污处理×表面相互作用（<0.0001），表明处理效果取决于应用处理的表面。在水泥表面中，次氯酸钠导致最大（<0.05）的循环阈值，其次是甲醛，与其余处理相比，甲醛具有更大（<0.05）的Ct值。在聚乙烯、橡胶和不锈钢表面中，经甲醛处理的表面具有最大的Ct值（<0.05），其次是次氯酸钠处理，其他处理的Ct值均较低（<0.05）。对于编织聚乙烯表面，与所有其他处理相比，甲醛和次氯酸钠处理具有更大的Ct值（<0.05）。有必要进行进一步的研究，以确定去污处理对PEDV传染性的作用，并开发饲料生产设施的去污方法。
