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山梨醇和过表达可减轻[具体物种]伴侣蛋白突变体中的温度敏感性。

Sorbitol and overexpression alleviate temperature sensitivity in chaperonin mutants of .

作者信息

Narayanan Aswathy, Kabir M Anaul

机构信息

School of Biotechnology, National Institute of Technology Calicut Kerala.

Molecular Mycology laboratory, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, Karnataka.

出版信息

MicroPubl Biol. 2021 Aug 5;2021. doi: 10.17912/micropub.biology.000440. eCollection 2021.

DOI:10.17912/micropub.biology.000440
PMID:34377964
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8343407/
Abstract

CCT (Chaperonin containing TCP-1) is a constitutively expressed eukaryotic chaperonin complex involved in the proper folding of proteins like actin and tubulin. Temperature sensitive mutants of CCT complex have been employed in various genetic screens, acting as models to study human CCT, the defects of which are implicated in disease conditions like neurodegeneration. Mutants of CCT complex are sensitive to cell wall stress agents. In this study, we have tested the effects of sorbitol and protein kinase C overexpression on the temperature sensitivity of mutants. We report that both the factors alleviated temperature sensitivity of mutants, indicating the possible role of CCT in maintaining cell wall integrity in .

摘要

包含TCP-1的伴侣蛋白（CCT）是一种组成型表达的真核伴侣蛋白复合体，参与肌动蛋白和微管蛋白等蛋白质的正确折叠。CCT复合体的温度敏感突变体已用于各种遗传筛选，作为研究人类CCT的模型，其缺陷与神经退行性变等疾病状态有关。CCT复合体的突变体对细胞壁应激剂敏感。在本研究中，我们测试了山梨醇和蛋白激酶C过表达对突变体温度敏感性的影响。我们报告这两个因素均减轻了突变体的温度敏感性，表明CCT在维持[具体物种]细胞壁完整性中可能发挥的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/8343407/eb280b798dbd/25789430-2021-micropub.biology.000440.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/8343407/eb280b798dbd/25789430-2021-micropub.biology.000440.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/947e/8343407/eb280b798dbd/25789430-2021-micropub.biology.000440.jpg

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本文引用的文献

1
Chaperonin TRiC/CCT supports mitotic exit and entry into endocycle in Drosophila.伴侣蛋白 TRiC/CCT 支持果蝇有丝分裂退出和进入内循环。
PLoS Genet. 2019 Apr 29;15(4):e1008121. doi: 10.1371/journal.pgen.1008121. eCollection 2019 Apr.
2
The TRiC chaperonin controls reovirus replication through outer-capsid folding.TRiC 伴侣蛋白通过外壳折叠控制呼肠孤病毒的复制。
Nat Microbiol. 2018 Apr;3(4):481-493. doi: 10.1038/s41564-018-0122-x. Epub 2018 Mar 12.
3
In Vivo Function of the Chaperonin TRiC in α-Actin Folding during Sarcomere Assembly.
在肌节组装过程中伴侣蛋白 TRiC 对α-肌动蛋白折叠的体内功能。
Cell Rep. 2018 Jan 9;22(2):313-322. doi: 10.1016/j.celrep.2017.12.069.
4
TRiC/CCT chaperonins are essential for maintaining myofibril organization, cardiac physiological rhythm, and lifespan.TRiC/CCT伴侣蛋白对于维持肌原纤维组织、心脏生理节律和寿命至关重要。
FEBS Lett. 2017 Nov;591(21):3447-3458. doi: 10.1002/1873-3468.12860. Epub 2017 Oct 10.
5
Defects in Protein Folding Machinery Affect Cell Wall Integrity and Reduce Ethanol Tolerance in S. cerevisiae.蛋白质折叠机制缺陷影响酿酒酵母的细胞壁完整性并降低其乙醇耐受性。
Curr Microbiol. 2016 Jul;73(1):38-45. doi: 10.1007/s00284-016-1024-x. Epub 2016 Mar 18.
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The cytosolic chaperonin CCT/TRiC and cancer cell proliferation.细胞质伴侣 CCT/TRiC 与癌细胞增殖。
PLoS One. 2013 Apr 16;8(4):e60895. doi: 10.1371/journal.pone.0060895. Print 2013.
7
Overexpressed ribosomal proteins suppress defective chaperonins in Saccharomyces cerevisiae.过表达的核糖体蛋白可抑制酿酒酵母中缺陷型伴侣蛋白。
FEMS Yeast Res. 2008 Dec;8(8):1236-44. doi: 10.1111/j.1567-1364.2008.00425.x. Epub 2008 Aug 1.
8
ATP-induced allostery in the eukaryotic chaperonin CCT is abolished by the mutation G345D in CCT4 that renders yeast temperature-sensitive for growth.CCT4 中的 G345D 突变消除了真核伴侣蛋白 CCT 中 ATP 诱导的变构作用，该突变使酵母对生长温度敏感。
J Mol Biol. 2008 Mar 21;377(2):469-77. doi: 10.1016/j.jmb.2008.01.011. Epub 2008 Jan 15.
9
The Hsp40 molecular chaperone Ydj1p, along with the protein kinase C pathway, affects cell-wall integrity in the yeast Saccharomyces cerevisiae.热休克蛋白40分子伴侣Ydj1p与蛋白激酶C途径一起，影响酿酒酵母的细胞壁完整性。
Genetics. 2007 Apr;175(4):1649-64. doi: 10.1534/genetics.106.066274. Epub 2007 Jan 21.
10
The chaperonin TRiC controls polyglutamine aggregation and toxicity through subunit-specific interactions.伴侣蛋白TRiC通过亚基特异性相互作用控制多聚谷氨酰胺聚集和毒性。
Nat Cell Biol. 2006 Oct;8(10):1155-62. doi: 10.1038/ncb1477. Epub 2006 Sep 17.