Programa de Pós-Graduação em Genética e Melhoramento, Universidade Federal do Espírito Santo, Laboratório de Bioquímica e Biologia Molecular, Centro de Ciências Agrárias e Engenharias, Alto Universitário, s/n, Guararema, 29500-000 Alegre, ES, Brazil.
Embrapa Café/ Incaper, Empresa Brasileira de Pesquisa Agropecuária/Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural, Rua Afonso Sarlo, 160, Bento Ferreira, 29052-010 Vitória, ES, Brazil.
An Acad Bras Cienc. 2021 Aug 9;93(suppl 3):e20201649. doi: 10.1590/0001-3765202120201649. eCollection 2021.
The objective of the present work was the molecular characterization of 11 parents and 101 hybrid progenies of conilon coffee, obtained through diallel crosses from the breeding program of the Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural (Incaper, ES, Brazil). The analyses were performed with 18 Simple Sequence Repeat (SSR) molecular markers, obtaining a total of 32 alleles. SSR markers were classified as moderately informative (PIC = 0.37), being efficient in characterizing individuals. High genetic diversity was verified in the 112 genotypes, based on the greater values of observed heterozygosity about to the expected heterozygosity (0.55 and 0.44, respectively), negative values for the fixation index (F) (-0.14), and the formation of distinct groups by UPGMA. These results indicate high genetic variability among the conilon coffee genitors, which remained similar and persisting in the progenies. The average dissimilarity between parents was 0.29 and between progenies 0.34. The progenies 38 and 40 and the parent P11 were considered the most divergent in the study. The genetic variability found can be explored in the genetic breeding of the conilon coffee and guide crossings between diversified and compatible genetic materials, for the composition of novel cultivars for the state of Espírito Santo.
本工作的目的是对来自巴西 ES 州 Incaper 研究所的咖啡品种康尼隆(conilon)的 11 个亲本和 101 个杂交后代进行分子特征分析。这些杂交后代是通过完全双列杂交得到的。分析采用了 18 个简单序列重复(SSR)分子标记,共得到 32 个等位基因。SSR 标记被归类为中度信息标记(PIC = 0.37),能够有效地对个体进行特征描述。在 112 个基因型中,观察到的杂合度(0.55)明显高于预期杂合度(0.44),固定指数(F)为负值(-0.14),UPGMA 聚类形成了不同的组,这表明基因型具有较高的遗传多样性。这些结果表明,康尼隆咖啡的亲本具有较高的遗传变异性,并且在后代中保持相似,持续存在。亲本之间的平均相似度为 0.29,后代之间的平均相似度为 0.34。在本研究中,后代 38 和 40 以及亲本 P11 被认为是最具差异的。所发现的遗传变异性可用于康尼隆咖啡的遗传育种,并指导多样化和相容的遗传材料之间的杂交,为圣埃斯皮里图州组成新的品种提供指导。