Jiangsu University of Science and Technology, School of Grain Science and Technology, Zhenjiang, P.R. China.
Braz J Biol. 2021 Aug 6;83:e243629. doi: 10.1590/1519-6984.243629. eCollection 2021.
As an important enzyme, xylanase is widely used in the food, pulp, and textile industry. Different applications of xylanase warrant specific conditions including temperature and pH. This study aimed to carry out sodium alginate beads as carrier to immobilize previous reported mutated xylanase from Neocallimastix patriciarum which expressed in E. coli, the activity of immobilization of mutated xylanase was elevated about 4% at pH 6 and 13% at 62 °C. Moreover, the immobilized mutated xylanase retained a greater proportion of its activity than the wide type in thermostability. These properties suggested that the immobilization of mutated xylanase has potential to apply in biobleaching industry.
作为一种重要的酶，木聚糖酶广泛应用于食品、纸浆和纺织工业。木聚糖酶的不同应用需要特定的条件，包括温度和 pH 值。本研究旨在利用海藻酸钠珠作为载体固定先前报道的来自 Neocallimastix patriciarum 的突变木聚糖酶，该突变木聚糖酶在大肠杆菌中表达，其固定化活性在 pH6 时提高了约 4%，在 62°C 时提高了 13%。此外，固定化的突变木聚糖酶比野生型在热稳定性方面保留了更大比例的活性。这些特性表明，突变木聚糖酶的固定化具有在生物漂白工业中应用的潜力。
