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内含子对小鼠L细胞中细胞胸苷激酶mRNA的有效形成无关紧要。

Introns are inconsequential to efficient formation of cellular thymidine kinase mRNA in mouse L cells.

作者信息

Gross M K, Kainz M S, Merrill G F

机构信息

Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.

出版信息

Mol Cell Biol. 1987 Dec;7(12):4576-81. doi: 10.1128/mcb.7.12.4576-4581.1987.

DOI:10.1128/mcb.7.12.4576-4581.1987
PMID:3437897
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368147/
Abstract

TK mRNA levels were determined in mouse L cells transformed with intron deletion mutations of the chicken TK gene. Whether normalized per cell, per integrated gene, or per internal control signal, intron deletion did not diminish the efficiency of TK mRNA formation in transformed L cells. The results demonstrated that introns are not required for efficient biogenesis of cellular mRNA in transformed mouse L cells.

摘要

在经鸡TK基因内含子缺失突变转化的小鼠L细胞中测定了TK mRNA水平。无论以每个细胞、每个整合基因还是每个内部对照信号进行标准化，内含子缺失都不会降低转化的L细胞中TK mRNA形成的效率。结果表明，在转化的小鼠L细胞中，高效生成细胞mRNA不需要内含子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e39/368147/d3ef45fc0822/molcellb00084-0439-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e39/368147/5825ababc54a/molcellb00084-0438-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e39/368147/26720dfa51f0/molcellb00084-0438-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e39/368147/d3ef45fc0822/molcellb00084-0439-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e39/368147/5825ababc54a/molcellb00084-0438-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e39/368147/26720dfa51f0/molcellb00084-0438-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e39/368147/d3ef45fc0822/molcellb00084-0439-a.jpg

相似文献

1
Introns are inconsequential to efficient formation of cellular thymidine kinase mRNA in mouse L cells.内含子对小鼠L细胞中细胞胸苷激酶mRNA的有效形成无关紧要。
Mol Cell Biol. 1987 Dec;7(12):4576-81. doi: 10.1128/mcb.7.12.4576-4581.1987.
2
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The sequence and context of the 5' splice site govern the nuclear stability of polyoma virus late RNAs.5'剪接位点的序列和上下文决定了多瘤病毒晚期RNA的核稳定性。
Nucleic Acids Res. 1995 Dec 11;23(23):4812-7. doi: 10.1093/nar/23.23.4812.
3
Stimulation of gene expression by introns: conversion of an inhibitory intron to a stimulatory intron by alteration of the splice donor sequence.内含子对基因表达的刺激作用：通过改变剪接供体序列将抑制性内含子转变为刺激性内含子。

本文引用的文献

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Synthesis of an unspliced cytoplasmic message by an adenovirus 5 deletion mutant.腺病毒5型缺失突变体合成未剪接的细胞质信使核糖核酸。
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Regulation of thymidine kinase protein levels during myogenic withdrawal from the cell cycle is independent of mRNA regulation.在肌源性细胞退出细胞周期过程中，胸苷激酶蛋白水平的调节独立于mRNA调节。
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在分化的肌肉细胞中，胸苷激酶（tk）酶的表达是通过细胞胸苷激酶基因的一个内部片段来调控的。
Mol Cell Biol. 1984 Sep;4(9):1777-84. doi: 10.1128/mcb.4.9.1777-1784.1984.
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Construction and functional characterization of polyomavirus genomes that separately encode the three early proteins.分别编码三种早期蛋白的多瘤病毒基因组的构建及功能表征。
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Splicing of adenovirus 2 early region 1A mRNAs is non-sequential.腺病毒2型早期区域1A信使核糖核酸的剪接是不连续的。
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Nature. 1981 Nov 19;294(5838):228-32. doi: 10.1038/294228a0.
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