小鼠细胞中染色体内部基因转换的准确性。
Accuracy of intrachromosomal gene conversion in mouse cells.
作者信息
Stachelek J L, Liskay R M
机构信息
Department of Therapeutic Radiology, Yale School of Medicine, New Haven, CT 06510.
出版信息
Nucleic Acids Res. 1988 May 11;16(9):4069-76. doi: 10.1093/nar/16.9.4069.
Abstract
Results of several recent studies suggest that homologous recombination and related processes in mammalian cells are highly mutagenic. We have examined the products of intrachromosomal gene conversion events that encompassed the last intron of the chicken thymidine kinase gene. Following plasmid rescue and DNA sequencing, we find no mutations associated with twenty conversion events representing 5380 total base pairs of which 2414 base pairs are intron sequence. Based on these studies we conclude that intrachromosomal gene conversion in mouse cells is not a highly mutagenic process but rather it operates with fidelity.
摘要
最近几项研究的结果表明，哺乳动物细胞中的同源重组及相关过程具有高度致突变性。我们检测了鸡胸苷激酶基因最后一个内含子的染色体内基因转换事件的产物。经过质粒拯救和DNA测序，我们发现，在代表5380个总碱基对（其中2414个碱基对为内含子序列）的20个转换事件中，未发现相关突变。基于这些研究，我们得出结论，小鼠细胞中的染色体内基因转换不是一个高度致突变的过程，而是以保真度进行操作。
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本文引用的文献
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Introns are inconsequential to efficient formation of cellular thymidine kinase mRNA in mouse L cells.内含子对小鼠L细胞中细胞胸苷激酶mRNA的有效形成无关紧要。
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Proc Natl Acad Sci U S A. 1987 Aug;84(15):5340-4. doi: 10.1073/pnas.84.15.5340.
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