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RNA聚合酶II转录因子H4TF-1和H4TF-2需要金属来结合特定的DNA序列。

RNA polymerase II transcription factors H4TF-1 and H4TF-2 require metal to bind specific DNA sequences.

作者信息

Dailey L, Roberts S B, Heintz N

机构信息

Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.

出版信息

Mol Cell Biol. 1987 Dec;7(12):4582-4. doi: 10.1128/mcb.7.12.4582-4584.1987.

DOI:10.1128/mcb.7.12.4582-4584.1987
PMID:3437898
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368148/
Abstract

Specific DNA-binding and in vitro transcription activities of H4TF-1 and H4TF-2 are inactivated by chelating agents. Binding activity is restored by addition of Zn2+, and H4TF-2 is also reactivated by Fe2+. In contrast, preformed factor-DNA complexes are resistant to chelators. Therefore, metal ions are a required component of the H4TF-1 and H4TF-2 DNA-binding domains.

摘要

H4TF-1和H4TF-2的特异性DNA结合及体外转录活性可被螯合剂灭活。通过添加Zn2+可恢复结合活性，并且Fe2+也可使H4TF-2重新激活。相比之下，预先形成的因子-DNA复合物对螯合剂具有抗性。因此，金属离子是H4TF-1和H4TF-2 DNA结合结构域的必需组成成分。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d31/368148/61675d00a35f/molcellb00084-0444-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d31/368148/c28ee7e08b43/molcellb00084-0443-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d31/368148/c11cf99fabef/molcellb00084-0443-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d31/368148/099484d22ae4/molcellb00084-0443-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d31/368148/61675d00a35f/molcellb00084-0444-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d31/368148/c28ee7e08b43/molcellb00084-0443-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d31/368148/c11cf99fabef/molcellb00084-0443-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d31/368148/099484d22ae4/molcellb00084-0443-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d31/368148/61675d00a35f/molcellb00084-0444-a.jpg

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