van Wijnen A J, Aziz F, Graña X, De Luca A, Desai R K, Jaarsveld K, Last T J, Soprano K, Giordano A, Lian J B
Department of Cell Biology, University of Massachusetts Medical School and Cancer Center, Worcester 01655.
Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12882-6. doi: 10.1073/pnas.91.26.12882.
Cell cycle-controlled human histone genes are coordinately expressed during S phase, and transcriptional regulation involves a series of trans-acting factors (HiNFs). The proliferation-specific factor HiNF-D interacts with multiple recognition motifs in histone H4, H3, and H1 promoters. Using gel shift immunoassays, we show that CDC2, cyclin A, and an RB-related protein are ubiquitous subunits of HiNF-D binding activity isolated from several cell types. HiNF-D levels in vivo are sensitive to okadaic acid and staurosporine, indicating that HiNF-D activity and/or assembly is influenced by phosphorylation status. Thus, HiNF-D appears to be a multicomponent phosphoprotein that participates in coordinate control of multiple histone H4, H3, and H1 genes during the cell cycle. The presence of cell cycle mediators in the HiNF-D complex suggests linkage between transcriptional control of histones, enzymes involved in DNA synthesis, and the onset of DNA replication during the G1/S phase transition.
细胞周期调控的人类组蛋白基因在S期协同表达，转录调控涉及一系列反式作用因子（HiNFs）。增殖特异性因子HiNF-D与组蛋白H4、H3和H1启动子中的多个识别基序相互作用。利用凝胶迁移免疫分析，我们发现CDC2、细胞周期蛋白A和一种RB相关蛋白是从几种细胞类型中分离出的HiNF-D结合活性的普遍亚基。体内HiNF-D水平对冈田酸和星形孢菌素敏感，表明HiNF-D活性和/或组装受磷酸化状态影响。因此，HiNF-D似乎是一种多组分磷蛋白，在细胞周期中参与多个组蛋白H4、H3和H1基因的协同控制。HiNF-D复合物中存在细胞周期介质，表明组蛋白转录调控、参与DNA合成的酶与G1/S期转换期间DNA复制起始之间存在联系。
