Graduate Course of Health and Social Services Master's Program, Saitama Prefectural University, Saitama, Japan.
National Institute for Communicable Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Centre for Disease Control and Prevention, Beijing, China.
J Clin Microbiol. 2021 Oct 19;59(11):e0015721. doi: 10.1128/JCM.00157-21. Epub 2021 Aug 11.
Legionella pneumophila, which is the main cause of Legionnaires' disease, comprises at least 15 serogroups (SGs). We show here the diversity of lipopolysaccharide biosynthetic loci among serogroups and describe the development of a PCR serotyping assay for 15 SGs based on the sequences of LPS biosynthetic loci. Using this multiplex-PCR (M-PCR) system, serogroups were detected using primers that specifically amplify the sequences of SG1, SG2, SG5, SG7, SG8, SG9, SG11, SG13, SG3/15, and SG6/12. When PCR products of the expected sizes were not detected, we used primers that identified SG4/10/14. The PCR serotyping system specifically amplified the sequences corresponding to SGs of 238 L. pneumophila strains. This method will be very useful for conducting epidemiological studies and investigating outbreak of Legionnaires' disease.
嗜肺军团菌是军团病的主要病原体，至少由 15 个血清群（SG）组成。我们在此展示了不同血清群中脂多糖生物合成基因座的多样性，并描述了基于 LPS 生物合成基因座序列开发用于 15 个 SG 的 PCR 血清分型检测方法。使用这种多重 PCR（M-PCR）系统，我们使用特异性扩增 SG1、SG2、SG5、SG7、SG8、SG9、SG11、SG13、SG3/15 和 SG6/12 序列的引物来检测血清群。当未检测到预期大小的 PCR 产物时，我们使用可识别 SG4/10/14 的引物。PCR 血清分型系统特异性扩增与 238 株嗜肺军团菌的 SG 相对应的序列。该方法将非常有助于开展流行病学研究和调查军团病的爆发。
