• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

COX10-AS1/miR-641/E2F6反馈环参与胶质瘤进展。

The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma.

作者信息

Liu Liang, Li Xiaojian, Wu Heming, Tang Yong, Li Xiang, Shi Yan

机构信息

Department of Neurosurgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.

出版信息

Front Oncol. 2021 Jul 26;11:648152. doi: 10.3389/fonc.2021.648152. eCollection 2021.

DOI:10.3389/fonc.2021.648152
PMID:34381702
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8350443/
Abstract

Glioma is the most common primary tumour of the central nervous system and is considered one of the greatest challenges for neurosurgery. Mounting evidence has shown that lncRNAs participate in various biological processes of tumours, including glioma. This study aimed to reveal the role and relevant mechanism of COX10-AS1 in glioma. The expression of COX10-AS1, miR-641 and E2F6 was measured by qRT-PCR and/or western blot. Clone formation assays, EdU assays, Transwell assays and tumour xenograft experiments were performed to evaluate the effects of COX10-AS1, miR-641 and E2F6 on glioma proliferation, migration and invasion. Luciferase reporter assays, RNA pull-down assays and ChIP assays were conducted to analyse the relationship among COX10-AS1, miR-641 and E2F6. We demonstrated that COX10-AS1 was upregulated in glioma tissues and cell lines, which was related to the grade of glioma and patient survival. Next, through functional assays, we found that COX10-AS1 influenced the proliferation, migration and invasion of glioma cell lines. Then, with the help of bioinformatics analysis, we confirmed that COX10-AS1 regulated glioma progress by acting as a sponge of miR-641 to regulate E2F6. Moreover, further study indicated that E2F6 could promote COX10-AS1 expression by binding to its promoter region. Taken together, the data indicated that COX10-AS1 acts as an oncogene in combination with COX10-AS1/miR-641/E2F6 in glioma, which may be beneficial to the diagnosis and treatment of glioma.

摘要

胶质瘤是中枢神经系统最常见的原发性肿瘤，被认为是神经外科面临的最大挑战之一。越来越多的证据表明，长链非编码RNA（lncRNAs）参与肿瘤的各种生物学过程，包括胶质瘤。本研究旨在揭示COX10-AS1在胶质瘤中的作用及相关机制。通过qRT-PCR和/或蛋白质免疫印迹法检测COX10-AS1、miR-641和E2F6的表达。进行克隆形成实验、EdU实验、Transwell实验和肿瘤异种移植实验，以评估COX10-AS1、miR-641和E2F6对胶质瘤增殖、迁移和侵袭的影响。进行荧光素酶报告基因实验、RNA下拉实验和染色质免疫沉淀实验，以分析COX10-AS1、miR-641和E2F6之间的关系。我们证明COX10-AS1在胶质瘤组织和细胞系中上调，这与胶质瘤的分级和患者生存有关。接下来，通过功能实验，我们发现COX10-AS1影响胶质瘤细胞系的增殖、迁移和侵袭。然后，借助生物信息学分析，我们证实COX10-AS1通过充当miR-641的海绵来调节E2F6，从而调控胶质瘤进展。此外，进一步研究表明，E2F6可通过结合其启动子区域促进COX10-AS1表达。综上所述，数据表明COX10-AS1在胶质瘤中与COX10-AS1/miR-641/E2F6结合发挥癌基因作用，这可能有助于胶质瘤的诊断和治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/3f2040e65075/fonc-11-648152-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/8e0408df86cb/fonc-11-648152-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/93cb8384b246/fonc-11-648152-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/5edd7a46bb2e/fonc-11-648152-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/917cf3219fc0/fonc-11-648152-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/cd2905df1156/fonc-11-648152-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/8cb6fc450b7f/fonc-11-648152-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/acb371f75315/fonc-11-648152-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/e5c5739889e8/fonc-11-648152-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/c596582550e2/fonc-11-648152-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/3f2040e65075/fonc-11-648152-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/8e0408df86cb/fonc-11-648152-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/93cb8384b246/fonc-11-648152-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/5edd7a46bb2e/fonc-11-648152-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/917cf3219fc0/fonc-11-648152-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/cd2905df1156/fonc-11-648152-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/8cb6fc450b7f/fonc-11-648152-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/acb371f75315/fonc-11-648152-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/e5c5739889e8/fonc-11-648152-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/c596582550e2/fonc-11-648152-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0786/8350443/3f2040e65075/fonc-11-648152-g010.jpg

相似文献

1
The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma.COX10-AS1/miR-641/E2F6反馈环参与胶质瘤进展。
Front Oncol. 2021 Jul 26;11:648152. doi: 10.3389/fonc.2021.648152. eCollection 2021.
2
Long Non-coding RNA COX10-AS1 Promotes Glioma Progression by Competitively Binding miR-1-3p to Regulate ORC6 Expression.长链非编码 RNA COX10-AS1 通过竞争性结合 miR-1-3p 来调节 ORC6 表达促进胶质瘤进展。
Neuroscience. 2024 Mar 5;540:68-76. doi: 10.1016/j.neuroscience.2023.09.020. Epub 2024 Jan 18.
3
COX10-AS1-mediated miR-361-5p regulated cell invasion and migration by targeting SPRY1 in oral squamous cell carcinoma.在口腔鳞状细胞癌中，COX10-AS1介导的miR-361-5p通过靶向SPRY1调控细胞侵袭和迁移。
Am J Transl Res. 2023 Mar 15;15(3):2191-2206. eCollection 2023.
4
Long non-coding RNA TP73-AS1 sponges miR-194 to promote colorectal cancer cell proliferation, migration and invasion via up-regulating TGFα.长链非编码 RNA TP73-AS1 通过海绵吸附 miR-194 促进结直肠癌细胞增殖、迁移和侵袭并上调 TGFα。
Cancer Biomark. 2018;23(1):145-156. doi: 10.3233/CBM-181503.
5
Knockdown of NCK1-AS1 inhibits the development of atherosclerosis by targeting miR-1197/COX10 axis.敲低NCK1-AS1通过靶向miR-1197/COX10轴抑制动脉粥样硬化的发展。
J Biol Eng. 2022 Jan 5;16(1):2. doi: 10.1186/s13036-021-00281-6.
6
Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410.长链非编码 RNA OIP5-AS1 通过调节 miR-410 靶向 Wnt-7b 影响胶质瘤的进展。
Biosci Rep. 2019 Jan 3;39(1). doi: 10.1042/BSR20180395. Print 2019 Jan 31.
7
COX10-AS1 Facilitates Cell Proliferation and Inhibits Cell Apoptosis in Glioblastoma Cells at Post-Transcription Level.COX10反义链1在转录后水平促进胶质母细胞瘤细胞增殖并抑制细胞凋亡。
Neurochem Res. 2020 Sep;45(9):2196-2203. doi: 10.1007/s11064-020-03081-4. Epub 2020 Aug 8.
8
MSC-AS1 knockdown inhibits cell growth and temozolomide resistance by regulating miR-373-3p/CPEB4 axis in glioma through PI3K/Akt pathway.MSC-AS1 通过调控 miR-373-3p/CPEB4 轴抑制 PI3K/Akt 通路抑制胶质瘤细胞生长和替莫唑胺耐药性
Mol Cell Biochem. 2021 Feb;476(2):699-713. doi: 10.1007/s11010-020-03937-x. Epub 2020 Oct 26.
9
Long non-coding RNA ZEB1-AS1 promotes glioma cell proliferation, migration and invasion through regulating miR-577.长链非编码 RNA ZEB1-AS1 通过调控 miR-577 促进胶质瘤细胞的增殖、迁移和侵袭。
Eur Rev Med Pharmacol Sci. 2018 May;22(10):3085-3093. doi: 10.26355/eurrev_201805_15068.
10
Long non-coding RNA SOX21-AS1 promotes cell proliferation and invasion through upregulating PAK7 expression by sponging miR-144-3p in glioma cells.长链非编码 RNA SOX21-AS1 通过海绵吸附 miR-144-3p 上调 PAK7 表达促进胶质瘤细胞增殖和侵袭。
Neoplasma. 2020 Mar;67(2):333-343. doi: 10.4149/neo_2020_190509N412. Epub 2020 Jan 21.

引用本文的文献

1
Silencing lncRNA GABPB1-AS1 alleviates cerebral ischemia reperfusion injury through the miR-641/NUCKS1 axis.沉默长链非编码RNA GABPB1-AS1通过miR-641/NUCKS1轴减轻脑缺血再灌注损伤。
Am J Transl Res. 2024 Jul 15;16(7):2963-2972. doi: 10.62347/EAGK7098. eCollection 2024.
2
Long noncoding RNA SNHG1 promotes breast cancer progression by regulating the miR-641/RRS1 axis.长链非编码 RNA SNHG1 通过调节 miR-641/RRS1 轴促进乳腺癌的进展。
Sci Rep. 2024 Feb 8;14(1):3265. doi: 10.1038/s41598-024-52953-0.
3
TBX15 facilitates malignant progression of glioma by transcriptional activation of TXDNC5.

本文引用的文献

1
The role of long non-coding RNA MIAT in cancers.长链非编码 RNA MIAT 在癌症中的作用。
Biomed Pharmacother. 2020 Sep;129:110359. doi: 10.1016/j.biopha.2020.110359. Epub 2020 Jun 11.
2
Editorial: Non-Coding RNAs and Human Diseases.社论：非编码RNA与人类疾病
Front Genet. 2020 May 25;11:523. doi: 10.3389/fgene.2020.00523. eCollection 2020.
3
Long Non-coding RNAs Involved in Pathogenic Infection.参与致病性感染的长链非编码RNA
TBX15通过转录激活TXDNC5促进胶质瘤的恶性进展。
iScience. 2024 Jan 18;27(2):108950. doi: 10.1016/j.isci.2024.108950. eCollection 2024 Feb 16.
4
Unveiling the Genetic Complexity of Teratozoospermia: Integrated Genomic Analysis Reveals Novel Insights into lncRNAs' Role in Male Infertility.揭示畸形精子症的遗传复杂性：综合基因组分析揭示 lncRNA 在男性不育中的作用的新见解。
Int J Mol Sci. 2023 Oct 9;24(19):15002. doi: 10.3390/ijms241915002.
5
The Role of Long Noncoding RNAs on Male Infertility: A Systematic Review and In Silico Analysis.长链非编码RNA在男性不育中的作用：系统评价与计算机分析
Biology (Basel). 2022 Oct 15;11(10):1510. doi: 10.3390/biology11101510.
6
Construction and Verification of a Novel Pyroptosis-Related lncRNA Signature Associated with Immune Landscape in Gliomas.一种与胶质瘤免疫格局相关的新型焦亡相关lncRNA特征的构建与验证
J Oncol. 2022 Oct 14;2022:7043431. doi: 10.1155/2022/7043431. eCollection 2022.
7
Effect of Nanoparticles of DOX and miR-125b on DNA Damage Repair in Glioma U251 Cells and Underlying Mechanisms.载多柔比星纳米粒和 miR-125b 对脑胶质瘤 U251 细胞 DNA 损伤修复的影响及其机制。
Molecules. 2022 Sep 21;27(19):6201. doi: 10.3390/molecules27196201.
8
A Biomimetic Platelet-Rich Plasma-Based Interpenetrating Network Printable Hydrogel for Bone Regeneration.一种用于骨再生的基于仿生富血小板血浆的互穿网络可打印水凝胶。
Front Bioeng Biotechnol. 2022 Apr 12;10:887454. doi: 10.3389/fbioe.2022.887454. eCollection 2022.
Front Genet. 2020 May 26;11:454. doi: 10.3389/fgene.2020.00454. eCollection 2020.
4
Screening and functional identification of lncRNAs in antler mesenchymal and cartilage tissues using high-throughput sequencing.利用高通量测序筛选和鉴定鹿茸间充质和软骨组织中的长链非编码 RNA。
Sci Rep. 2020 Jun 11;10(1):9492. doi: 10.1038/s41598-020-66383-1.
5
Identification of long noncoding RNAs involved in adaptability to chronic hypoxic by whole transcriptome sequencing.通过全转录组测序鉴定参与慢性缺氧适应性的长链非编码RNA
3 Biotech. 2020 Jun;10(6):269. doi: 10.1007/s13205-020-02272-8. Epub 2020 May 27.
6
SP1-Induced Upregulation of lncRNA LINC00514 Promotes Tumor Proliferation and Metastasis in Osteosarcoma by Regulating miR-708.SP1诱导的lncRNA LINC00514上调通过调控miR-708促进骨肉瘤肿瘤增殖和转移。
Cancer Manag Res. 2020 May 11;12:3311-3322. doi: 10.2147/CMAR.S242464. eCollection 2020.
7
Metformin Suppresses the Proliferation and Promotes the Apoptosis of Colon Cancer Cells Through Inhibiting the Expression of Long Noncoding RNA-UCA1.二甲双胍通过抑制长链非编码RNA-UCA1的表达抑制结肠癌细胞增殖并促进其凋亡。
Onco Targets Ther. 2020 May 14;13:4169-4181. doi: 10.2147/OTT.S245091. eCollection 2020.
8
A potential prognostic prediction model of colon adenocarcinoma with recurrence based on prognostic lncRNA signatures.基于预后长链非编码 RNA 特征的结肠癌复发的潜在预后预测模型。
Hum Genomics. 2020 Jun 10;14(1):24. doi: 10.1186/s40246-020-00270-8.
9
Construction of a metastasis-associated ceRNA network reveals a prognostic signature in lung cancer.构建转移相关的竞争性内源RNA网络揭示肺癌的预后特征
Cancer Cell Int. 2020 Jun 3;20:208. doi: 10.1186/s12935-020-01295-8. eCollection 2020.
10
MIR205HG acts as a ceRNA to expedite cell proliferation and progression in lung squamous cell carcinoma via targeting miR-299-3p/MAP3K2 axis.MIR205HG 通过靶向 miR-299-3p/MAP3K2 轴促进肺鳞癌细胞增殖和进展。
BMC Pulm Med. 2020 Jun 8;20(1):163. doi: 10.1186/s12890-020-1174-2.