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利用脑片培养技术对转基因胚胎鼠大脑中的小胶质细胞与放射状胶质细胞的相互作用进行活细胞成像。

Live-cell imaging of microglial interactions with radial glia in transgenic embryonic mouse brains using slice culture.

机构信息

Department of Medical Genetics, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada.

Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB T2N 4N1, Canada.

出版信息

STAR Protoc. 2021 Jul 29;2(3):100670. doi: 10.1016/j.xpro.2021.100670. eCollection 2021 Sep 17.

DOI:10.1016/j.xpro.2021.100670
PMID:34382012
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8339326/
Abstract

Microglial dynamics and interactions with nearby radial glia can be visualized in real time in embryonic mouse brain tissue using time-lapse imaging in slice culture. This live-cell imaging protocol can be used to study the morphology and activities of a number of cell types across a variety of brain regions and developmental time points. The advantage of this brain slice culture model is that it allows for the visualization of cellular interactions and movements in real time, especially across embryogenesis. For complete details on the use and execution of this protocol, please refer to Rosin et al. (2021).

摘要

使用切片培养中的延时成像，可以实时观察胚胎鼠脑组织中小胶质细胞的动态及其与附近放射状胶质细胞的相互作用。该活细胞成像方案可用于研究各种脑区和发育时间点的多种细胞类型的形态和活动。该脑片培养模型的优势在于可以实时观察细胞相互作用和运动，特别是在胚胎发生过程中。有关该方案使用和执行的完整详细信息，请参见 Rosin 等人（2021 年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/0fa9e7562bfb/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/a94d6b2fda6b/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/a30becdce43a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/83bc960b7af1/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/7c51c6761007/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/19948df08a1d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/69fd62e77e74/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/912de99b6952/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/967da3360902/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/0fa9e7562bfb/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/a94d6b2fda6b/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/a30becdce43a/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/83bc960b7af1/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/7c51c6761007/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/19948df08a1d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/69fd62e77e74/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/912de99b6952/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/967da3360902/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/99e8/8339326/0fa9e7562bfb/gr6.jpg

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Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia.异常表达微管相关蛋白 Tau 的存活神经元暴露磷脂酰丝氨酸并被小胶质细胞吞噬。
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J Neuroinflammation. 2018 Jun 12;15(1):181. doi: 10.1186/s12974-018-1213-6.
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