1Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India.
Plant RNAi Biology Group, ICGEB, New Delhi, 110067, India.
Arch Virol. 2021 Oct;166(10):2905-2909. doi: 10.1007/s00705-021-05179-8. Epub 2021 Aug 12.
Golden trumpet (Allamanda cathartica) plants were observed to exhibit mottling and distortion symptoms on leaves. The genome of an associated begomovirus (Al-K1) was amplified by rolling-circle amplification, cloned, and sequenced. The viral genome consisted of two circular ssDNA molecules, and the organization of the ORFs was similar to those of DNA-A and DNA-B components of bipartite begomoviruses. The size of DNA-A (KC202818) and DNA-B (MG969497) of the begomovirus was 2772 and 2690 nucleotides, respectively. Sequence analysis revealed that the DNA-A and DNA-B components shared the highest sequence identity with duranta leaf curl virus (MN537564, 87.8%) and cotton leaf curl Alabad virus (MH760452, 81.0%), respectively. Interestingly, the Al-K1 isolate shared significantly less nucleotide sequence identity with allamanda leaf curl virus (EF602306, 71.6%), the only monopartite begomovirus reported previously in golden trumpet from China. Al-K1 shared less than 91% sequence identity with other begomoviruses, and hence, according to the latest ICTV guidelines for species demarcation of begomoviruses, Al-K1 is proposed to be a member of a new species, and we propose the name "allamanda leaf mottle distortion virus" (AllLMoDV-[IN-Al_K1-12]) for this virus. AllLMoDV was detected in various golden trumpet samples from different locations by PCR with specific primers based on the genome sequence determined in this study. Our study provides evidence of the occurrence of a new bipartite begomovirus in a perennial ornamental plant in India.
金喇叭（Allamanda cathartica）植株叶片出现斑驳和畸形症状。通过滚环扩增、克隆和测序，扩增出与之相关的曲叶病毒（Al-K1）的基因组。病毒基因组由两个圆形 ssDNA 分子组成，ORF 的组织与双分体伴生病毒的 DNA-A 和 DNA-B 组分相似。该伴生病毒的 DNA-A（KC202818）和 DNA-B（MG969497）大小分别为 2772 和 2690 个核苷酸。序列分析表明，DNA-A 和 DNA-B 组分与钝叶罗勒卷曲病毒（MN537564，87.8%）和棉花叶卷曲阿拉巴德病毒（MH760452，81.0%）的序列同源性最高。有趣的是，Al-K1 分离株与先前在中国金喇叭中报道的唯一单分体曲叶病毒（EF602306，71.6%）的核苷酸序列同源性显著较低。Al-K1 与其他伴生病毒的序列同源性低于 91%，因此，根据最新的 ICTV 伴生病毒物种划分指南，Al-K1 被提议为一个新种的成员，我们建议将这种病毒命名为“金喇叭叶斑驳畸形病毒”（AllLMoDV-[IN-Al_K1-12]）。根据本研究确定的基因组序列，利用基于特异性引物的 PCR 技术在来自不同地点的各种金喇叭样本中检测到了 AllLMoDV。本研究为印度一种多年生观赏植物中发生新的双分体伴生病毒提供了证据。
