Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.
Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, 571101, China.
Arch Virol. 2024 May 16;169(6):124. doi: 10.1007/s00705-024-06041-3.
Allamanda cathartica is an ornamental medicinal plant that grows widely in the tropics. In the present study, two novel viruses, Allamanda chlorotic virus A (AlCVA) and Allamanda chlorotic virus B (AlCVB), were identified in an A. cathartica plant with interveinal chlorosis by ribosomal RNA-depleted total-RNA sequencing. Phylogenetic analysis and sequence comparisons confirmed that AlCVA and AlCVB belong to the families Closteroviridae and Betaflexiviridae, respectively. Long, flexuous, filamentous virus particles approximately 12 nm in diameter and 784-2291 nm in length were observed using transmission electron microscopy. A specific RT-PCR assay was used to demonstrate a consistent association of viral infection with symptoms.
长春花是一种广泛生长在热带地区的观赏药用植物。在本研究中，通过核糖体 RNA 耗尽的总 RNA 测序，从一株具脉间褪绿症状的长春花中鉴定出两种新型病毒，即长春花褪绿病毒 A（AlCVA）和长春花褪绿病毒 B（AlCVB）。系统进化分析和序列比较证实 AlCVA 和 AlCVB 分别属于杆状病毒科和贝塔病毒科。电镜观察到直径约 12nm、长 784-2291nm 的长而弯曲的丝状病毒粒子。利用 RT-PCR 检测证实了病毒感染与症状之间存在一致性关联。
