Postgraduate Program of Basic and Applied Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto-SP, Brazil.
Postgraduate Program of Pharmaceutical Sciences, Federal University of Rio Grande do Norte, Natal-RN, Brazil.
Arch Med Res. 2022 Feb;53(2):163-169. doi: 10.1016/j.arcmed.2021.07.004. Epub 2021 Aug 9.
Interleukin (IL)-17A has a dual role in tumor immunity, promotes anti-tumor responses and facilitates angiogenesis by interacting with IL-17 receptor A (IL-17RA). Although IL-17A has been associated with the pathogenesis of papillary thyroid carcinoma (PTC), the nucleotide variability at the IL17A and IL17RA genes is still poorly characterized.
To assess the contribution of the IL17A (-197 G >A, rs2275913) and IL17RA (-947 A >G, rs4819554) single nucleotide polymorphisms (SNP) on the development and progression of PTC and on IL-17 plasma levels.
We studied 188 PTC patients and 170 healthy controls. SNPs were identified using PCR-amplified DNA and restriction fragment length polymorphism (RFLP) techniques. Plasma levels of IL-17A was evaluated in 83 PTC patients using ELISA. Statistical analyses were performed to evaluate the associations between SNPs and clinicohistopathological features of PTC and IL-17A levels.
No significant difference was observed regarding the allele and genotype distributions of both SNPs between PTC patients and controls. The IL17A GA was associated with poor biochemical and structural incomplete response to therapy, whereas no influence over the IL-17A expression was observed. The IL17RA AG was significantly associated with small-sized tumors, initial tumor stage at diagnosis and better response to therapy.
The IL17A SNP may predict an aggressive manifestation of PTC, whereas the IL17RA SNP was associated with a more favorable clinical outcome.
白细胞介素（IL）-17A 在肿瘤免疫中具有双重作用，通过与 IL-17 受体 A（IL-17RA）相互作用，促进抗肿瘤反应并促进血管生成。尽管 IL-17A 与甲状腺乳头状癌（PTC）的发病机制有关，但 IL17A 和 IL17RA 基因的核苷酸变异性仍知之甚少。
评估 IL17A（-197 G>A，rs2275913）和 IL17RA（-947 A>G，rs4819554）单核苷酸多态性（SNP）对 PTC 发生和进展以及 IL-17 血浆水平的影响。
我们研究了 188 例 PTC 患者和 170 例健康对照者。使用聚合酶链反应扩增的 DNA 和限制性片段长度多态性（RFLP）技术鉴定 SNP。使用 ELISA 检测 83 例 PTC 患者的 IL-17A 血浆水平。统计分析用于评估 SNP 与 PTC 的临床病理特征和 IL-17A 水平之间的关系。
在 PTC 患者和对照组中，两种 SNP 的等位基因和基因型分布均无显著差异。IL17A GA 与不良生化和结构不完全治疗反应相关，而对 IL-17A 表达无影响。IL17RA AG 与肿瘤体积小、诊断时初始肿瘤分期和更好的治疗反应显著相关。
IL17A SNP 可能预测 PTC 的侵袭性表现，而 IL17RA SNP 与更有利的临床结局相关。
