Lee Young Chan, Chung Joo-Ho, Kim Su Kang, Rhee Sang Youl, Chon Suk, Oh Seung Joon, Hong Il Ki, Eun Young Gyu
Department of Otolaryngology-Head and Neck Surgery, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.
Kohwang Medical Research Institute, Kyung Hee University, School of Medicine, Seoul, Republic of Korea.
Cytokine. 2015 Feb;71(2):283-8. doi: 10.1016/j.cyto.2014.11.011. Epub 2014 Dec 5.
Although numerous recent studies have implicated a role for interleukin 17(IL17) in tumor development, the mechanisms of IL17 involvement are still uncharacterized. The aims of this study were to determine whether single nucleotide polymorphisms (SNPs) in IL17 and IL17R contribute to the development of papillary thyroid cancer (PTC) and to assess the relationship between IL17 and IL17R SNPs and the clinicopathologic characteristics of PTC.
Eight SNPs located within the IL17A, IL17RA, and IL17RB genes were genotyped using direct sequencing in 94 patients with PTC and 260 patients without PTC (controls). Genetic data were analyzed using commercially available software. Statistical analyses were then performed to examine the relationships between these SNPs and the clinicopathologic characteristics of PTC.
Genotyping analysis demonstrated that the IL17RA SNP rs4819554 (codominant model 1, odds ratio (OR)=0.39, P=0.001) and the IL17RB SNP rs1025689 (dominant model, OR=0.59, P=0.043) were significantly associated with lack of PTC. Interestingly, the IL17A SNP rs2275913 (codominant model 2, OR=0.19, P=0.034) was significantly associated with lack of multifocality. Furthermore, the IL17RA SNP rs4819554 (dominant model, OR=0.25, P=0.010) was significantly associated with lack of cancer bilaterality.
In this study of SNPs in the IL17 and IL17R genes in patients with PTC, we demonstrated that IL17RA polymorphisms can influence both the development and the bilaterality of PTC.
尽管近期大量研究表明白细胞介素17（IL17）在肿瘤发展中发挥作用，但其具体机制仍不清楚。本研究旨在确定IL17和IL17R基因的单核苷酸多态性（SNP）是否与甲状腺乳头状癌（PTC）的发生有关，并评估IL17和IL17R基因SNP与PTC临床病理特征之间的关系。
采用直接测序法对94例PTC患者和260例非PTC患者（对照组）的IL17A、IL17RA和IL17RB基因中的8个SNP进行基因分型。使用商业软件分析遗传数据。随后进行统计分析，以研究这些SNP与PTC临床病理特征之间的关系。
基因分型分析表明，IL17RA基因SNP rs4819554（共显性模型1，比值比（OR）=0.39，P=0.001）和IL17RB基因SNP rs1025689（显性模型，OR=0.59，P=0.043）与PTC的缺失显著相关。有趣的是，IL17A基因SNP rs2275913（共显性模型2，OR=0.19，P=0.034）与缺乏多灶性显著相关。此外，IL17RA基因SNP rs4819554（显性模型，OR=0.25，P=0.010）与缺乏双侧癌显著相关。
在本研究中，我们对PTC患者的IL17和IL17R基因SNP进行了分析，结果表明IL17RA基因多态性可影响PTC的发生和双侧性。
