植物中的碱基编辑:应用、挑战与未来前景

Base Editing in Plants: Applications, Challenges, and Future Prospects.

作者信息

Azameti Mawuli K, Dauda Wadzani Palnam

机构信息

National Institute for Plant Biotechnology, New Delhi, India.

Indian Agricultural Research Institute, New Delhi, India.

出版信息

Front Plant Sci. 2021 Jul 27;12:664997. doi: 10.3389/fpls.2021.664997. eCollection 2021.

Abstract

The ability to create targeted modifications in the genomes of plants using genome editing technologies has revolutionized research in crop improvement in the current dispensation of molecular biology. This technology has attracted global attention and has been employed in functional analysis studies in crop plants. Since many important agronomic traits are confirmed to be determined by single-nucleotide polymorphisms, improved crop varieties could be developed by the programmed and precise conversion of targeted single bases in the genomes of plants. One novel genome editing approach which serves for this purpose is base editing. Base editing directly makes targeted and irreversible base conversion without creating double-strand breaks (DSBs). This technology has recently gained quick acceptance and adaptation because of its precision, simplicity, and multiplex capabilities. This review focuses on generating different base-editing technologies and how efficient they are in editing nucleic acids. Emphasis is placed on the exploration and applications of these base-editing technologies to enhance crop production. The review also highlights the drawbacks and the prospects of this new technology.

摘要

在当前分子生物学时代,利用基因组编辑技术在植物基因组中进行靶向修饰的能力彻底改变了作物改良研究。这项技术已引起全球关注,并已应用于作物功能分析研究。由于许多重要的农艺性状已被证实由单核苷酸多态性决定,因此可以通过对植物基因组中靶向单碱基进行程序化和精确转换来培育改良作物品种。一种用于此目的的新型基因组编辑方法是碱基编辑。碱基编辑直接进行靶向且不可逆的碱基转换,而不会产生双链断裂(DSB)。这项技术因其精确性、简便性和多重编辑能力,最近迅速得到认可和应用。本综述重点介绍了不同碱基编辑技术的产生及其编辑核酸的效率。重点在于这些碱基编辑技术在提高作物产量方面的探索和应用。本综述还强调了这项新技术的缺点和前景。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6608/8353127/647ceb530e9c/fpls-12-664997-g001.jpg

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