• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过核磁共振弛豫测量研究牛超氧化物歧化酶中铜位点的性质。

Properties of the cupric sites in bovine superoxide dismutase studied by nuclear-magnetic-relaxation measurements.

作者信息

Boden N, Holmes M C, Knowles P F

出版信息

Biochem J. 1979 Jan 1;177(1):303-9. doi: 10.1042/bj1770303.

DOI:10.1042/bj1770303
PMID:34390
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186369/
Abstract

Proton nuclear-relaxation rates have been measured as a function of frequency, temperature, pH and cyanide concentration in aqueous solutions of superoxide dismutase from bovine erythrocytes. The results show that, whereas for pH less than or equal to 9 only one water molecule is bound to each Cu2+ ion, at higher pH a second co-ordination site for OH- becomes available; it is proposed that this involves cleavage of the bond between Cu2+ and the histidine residue that bridges to Zn2+.

摘要

已测量了来自牛红细胞的超氧化物歧化酶水溶液中质子核弛豫率随频率、温度、pH值和氰化物浓度的变化。结果表明，当pH值小于或等于9时，每个Cu2+离子仅结合一个水分子，而在较高pH值下，OH-的第二个配位位点变得可用；有人提出，这涉及到Cu2+与桥连到Zn2+的组氨酸残基之间的键断裂。

相似文献

1
Properties of the cupric sites in bovine superoxide dismutase studied by nuclear-magnetic-relaxation measurements.通过核磁共振弛豫测量研究牛超氧化物歧化酶中铜位点的性质。
Biochem J. 1979 Jan 1;177(1):303-9. doi: 10.1042/bj1770303.
2
The binding of copper ions to copper-free bovine superoxide dismutase. Properties of the protein recombined with increasing amounts of copper ions.铜离子与无铜牛超氧化物歧化酶的结合。随着铜离子含量增加而重组的蛋白质的性质。
Biochem J. 1977 Jan 1;161(1):31-5. doi: 10.1042/bj1610031.
3
The binding of copper ions to copper-free bovine superoxide dismutase. Kinetic aspects.铜离子与无铜牛超氧化物歧化酶的结合。动力学方面。
Biochem J. 1978 Feb 1;169(2):277-80. doi: 10.1042/bj1690277.
4
An extended X-ray-absorption-fine-structure study of the copper and zinc sites of freeze-dried bovine superoxide dismutase.冻干牛超氧化物歧化酶铜和锌位点的扩展X射线吸收精细结构研究
Biochem J. 1983 Sep 1;213(3):765-8. doi: 10.1042/bj2130765.
5
The binding of copper ions to copper-free bovine superoxide dismutase. Copper distribution in protein samples recombined with less than stoicheiometric copper ion/protein ratios.铜离子与无铜牛超氧化物歧化酶的结合。在铜离子/蛋白质比例低于化学计量比的情况下重新组合的蛋白质样品中的铜分布。
Biochem J. 1977 Jan 1;161(1):27-30. doi: 10.1042/bj1610027.
6
Studies on the reconstituion of bovine erythrocyte superoxide dismutase. 3. Evidence for a strong interdependence between Cu 2+ and Zn 2+ binding in the expression of the spectroscopic properties of the native protein and for a close proximity of the Zn 2+ and Cu 2+ sites.牛红细胞超氧化物歧化酶重组研究。3. 天然蛋白质光谱性质表达中铜离子（Cu²⁺）与锌离子（Zn²⁺）结合之间存在强烈相互依赖性以及锌离子和铜离子位点紧密相邻的证据。
Biochim Biophys Acta. 1973 Jan 25;295(1):107-16.
7
Imidazolate bridged Cu(II)-Cu(II) and Cu(II)-Zn(II) complexes of a terpyridinophane azamacrocycle: a solution and solid state study.三联吡啶环番氮杂大环的咪唑盐桥联铜（II）-铜（II）和铜（II）-锌（II）配合物：溶液和固态研究
Dalton Trans. 2007 Nov 7(41):4726-37. doi: 10.1039/b708186c. Epub 2007 Aug 30.
8
The exchange of histidine C-2 protons in superoxide dismutases. A novel method for assigning histidine-metal ligands in proteins.超氧化物歧化酶中组氨酸C-2质子的交换。一种确定蛋白质中组氨酸-金属配体的新方法。
Biochem J. 1979 Oct 1;183(1):127-32. doi: 10.1042/bj1830127.
9
Crystal structure of the cyanide-inhibited Xenopus laevis Cu,Zn superoxide dismutase at 98 K.
FEBS Lett. 1994 Jul 25;349(1):93-8. doi: 10.1016/0014-5793(94)00651-2.
10
The crystal structure of bovine Cu2+,Zn2+ superoxide dismutase at 5.5-A resolution.
J Biol Chem. 1974 Sep 10;249(17):5677-83.

引用本文的文献

1
Comparative kinetic study between native and chemically modified Cu,Zn superoxide dismutases.天然与化学修饰的铜锌超氧化物歧化酶的比较动力学研究
Biochem J. 1993 Jun 1;292 ( Pt 2)(Pt 2):451-5. doi: 10.1042/bj2920451.
2
An extended-X-ray-absorption-fine-structure study of bovine erythrocyte superoxide dismutase in aqueous solution. Direct evidence for three-co-ordinate Cu(I) in reduced enzyme.水溶液中牛红细胞超氧化物歧化酶的扩展X射线吸收精细结构研究。还原态酶中三配位Cu(I)的直接证据。
Biochem J. 1984 May 1;219(3):985-90. doi: 10.1042/bj2190985.
3
Kinetic and e.p.r. studies of cyanide and azide binding to the copper sites of dopamine (3,4-dihydroxyphenethylamine) beta-mono-oxygenase.氰化物和叠氮化物与多巴胺（3,4-二羟基苯乙胺）β-单加氧酶铜位点结合的动力学和电子顺磁共振研究。
Biochem J. 1984 Jun 1;220(2):447-54. doi: 10.1042/bj2200447.
4
The effects of pH and various salts upon the activity of a series of superoxide dismutases.pH值和各种盐类对一系列超氧化物歧化酶活性的影响。
Biochem J. 1988 Apr 1;251(1):41-6. doi: 10.1042/bj2510041.
5
An investigation of Cu2Zn2 superoxide dismutase and its Ile-137 mutant at high pH.高pH条件下Cu2Zn2超氧化物歧化酶及其Ile-137突变体的研究。
Eur Biophys J. 1991;19(3):141-6. doi: 10.1007/BF00185454.
6
Properties of cupric ions in benzylamine oxidase from pig plasma as studied by magnetic-resonance and kinetic methods.通过磁共振和动力学方法研究猪血浆中苄胺氧化酶中铜离子的性质。
Biochem J. 1979 Jan 1;177(1):289-302. doi: 10.1042/bj1770289.

本文引用的文献

1
Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).超氧化物歧化酶。红细胞铜蛋白（血铜蛋白）的酶功能。
J Biol Chem. 1969 Nov 25;244(22):6049-55.
2
Nuclear magnetic relaxation dispersion in protein solutions. V. Bovine erythrocyte superoxide dismutase.
Biochim Biophys Acta. 1972 Jun 22;271(1):1-5. doi: 10.1016/0005-2795(72)90126-2.
3
Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.超氧化物歧化酶：改进的测定方法及一种适用于丙烯酰胺凝胶的测定方法。
Anal Biochem. 1971 Nov;44(1):276-87. doi: 10.1016/0003-2697(71)90370-8.
4
pH dependece of the nuclear magnetic relaxation rate of solvent water protons in solutions of bovine superoxide dismutase.牛超氧化物歧化酶溶液中溶剂水质子的核磁共振弛豫率与pH的关系
Biochim Biophys Acta. 1974 Jun 7;351(2):230-6. doi: 10.1016/0005-2795(74)90185-8.
5
Binding of water to "types I and II" Cu2+ in proteins.水与蛋白质中“Ⅰ型和Ⅱ型”Cu2+的结合。
Biochem Biophys Res Commun. 1974 Apr 8;57(3):845-8. doi: 10.1016/0006-291x(74)90623-8.
6
Mechanism of action of superoxide dismutase from pulse radiolysis and electron paramagnetic resonance. Evidence that only half the active sites function in catalysis.脉冲辐解和电子顺磁共振研究超氧化物歧化酶的作用机制。证据表明只有一半的活性位点参与催化作用。
Biochem J. 1974 Apr;139(1):49-60. doi: 10.1042/bj1390049.
7
Studies of the metal sites of copper proteins. Symmetry of copper in bovine superoxide dismutase and its functional significance.
Biochemistry. 1972 May 23;11(11):2187-92. doi: 10.1021/bi00761a028.
8
Anion binding to bovine erythrocyte superoxide dismutase. Evidence for multiple binding sites with qualitatively different properties.阴离子与牛红细胞超氧化物歧化酶的结合。具有性质不同的多个结合位点的证据。
J Biol Chem. 1972 Jan 10;247(1):60-5.
9
Superoxide dismutases.超氧化物歧化酶
Annu Rev Biochem. 1975;44:147-59. doi: 10.1146/annurev.bi.44.070175.001051.
10
Crystal structure of bovine Cu,Zn superoxide dismutase at 3 A resolution: chain tracing and metal ligands.
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1349-53. doi: 10.1073/pnas.72.4.1349.