通过核磁共振弛豫测量研究牛超氧化物歧化酶中铜位点的性质。
Properties of the cupric sites in bovine superoxide dismutase studied by nuclear-magnetic-relaxation measurements.
作者信息
Boden N, Holmes M C, Knowles P F
出版信息
Biochem J. 1979 Jan 1;177(1):303-9. doi: 10.1042/bj1770303.
Abstract
Proton nuclear-relaxation rates have been measured as a function of frequency, temperature, pH and cyanide concentration in aqueous solutions of superoxide dismutase from bovine erythrocytes. The results show that, whereas for pH less than or equal to 9 only one water molecule is bound to each Cu2+ ion, at higher pH a second co-ordination site for OH- becomes available; it is proposed that this involves cleavage of the bond between Cu2+ and the histidine residue that bridges to Zn2+.
摘要
已测量了来自牛红细胞的超氧化物歧化酶水溶液中质子核弛豫率随频率、温度、pH值和氰化物浓度的变化。结果表明，当pH值小于或等于9时，每个Cu2+离子仅结合一个水分子，而在较高pH值下，OH-的第二个配位位点变得可用；有人提出，这涉及到Cu2+与桥连到Zn2+的组氨酸残基之间的键断裂。
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Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).超氧化物歧化酶。红细胞铜蛋白（血铜蛋白）的酶功能。
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pH dependece of the nuclear magnetic relaxation rate of solvent water protons in solutions of bovine superoxide dismutase.牛超氧化物歧化酶溶液中溶剂水质子的核磁共振弛豫率与pH的关系
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