Winger C M, Canning E U, Culverhouse J D
Department of Biology, Imperial College, London, Gt. Britain.
Vet Parasitol. 1987 Dec;26(1-2):43-53. doi: 10.1016/0304-4017(87)90075-6.
Immunogens derived from microaerophilous stationary phase (MASP) cultures of Babesia divergens grown in bovine erythrocytes were used to inoculate the laboratory host of B. divergens, the Mongolian gerbil, Meriones unguiculatus. Animals inoculated subcutaneously twice with preparations of freeze-thawed merozoites in complete Freund's adjuvant were fully protected against homologous challenge, as were gerbils immunised with a non-viable preparation of parasite-enriched lysed infected bovine erythrocytes. Animals which had been infected with small numbers of parasitised erythrocytes from cultures cooled to 4 degrees C, allowed to recover, then challenged, also survived. All three groups had high antibody titres which dropped immediately after challenge and then rose again. Gerbils given culture supernatants containing soluble merozoite protein coat antigens were partially protected only after receiving a third inoculation. Non-immunised animals all died 4 days after challenge.
用在牛红细胞中生长的微小牛巴贝斯虫微需氧静止期（MASP）培养物衍生的免疫原接种微小牛巴贝斯虫的实验宿主蒙古沙鼠（Meriones unguiculatus）。用冻融裂殖子制剂在完全弗氏佐剂中皮下接种两次的动物，对同源攻击具有完全保护作用，用富含寄生虫的裂解感染牛红细胞的无活力制剂免疫的沙鼠也是如此。感染了少量来自冷却至4摄氏度培养物的寄生红细胞、恢复后再接受攻击的动物也存活了下来。所有三组动物的抗体滴度都很高，在攻击后立即下降，然后又再次上升。仅在接受第三次接种后，给予含有可溶性裂殖子蛋白包膜抗原的培养上清液的沙鼠才得到部分保护。未免疫的动物在攻击后4天全部死亡。
