South China Advanced Institute for Soft Matter Science and Technology, School of Molecular Science and Engineering, Guangdong Provincial Key Laboratory of Functional and Intelligent Hybrid Materials and Devices, South China University of Technology, Guangzhou 510640, China.
South China Advanced Institute for Soft Matter Science and Technology, School of Molecular Science and Engineering, Guangdong Provincial Key Laboratory of Functional and Intelligent Hybrid Materials and Devices, South China University of Technology, Guangzhou 510640, China.
J Colloid Interface Sci. 2022 Jan 15;606(Pt 1):248-260. doi: 10.1016/j.jcis.2021.08.021. Epub 2021 Aug 8.
Regulating cell behavior and function by surface topography has drawn significant attention in tissue engineering. Herein, a gradient fibrous scaffold comprising anisotropic aligned fibers and isotropic annealed fibers was developed to provide a controllable direction of cell migration, adhesion, and spreading. The electrospun aligned fibers were engraved to create surface gradients with micro-and-nanometer roughness through block copolymer (BCP) self-assembly induced by selective solvent vapor annealing (SVA). The distinct manipulation of cell behavior by annealed fibrous scaffolds with tailored self-assembled nanostructure and welded fibrous microstructure has been illustrated by in situ/ex situ small angle X-ray scattering (SAXS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and in vitro cell culture. Further insights into the effect of integrated gradient fibrous scaffold were gained at the level of protein expression. From the perspective of gradient topology, this region-specific scaffold based on BCP fibers shows the prospect of guiding cell migration, adhesion and spreading and provides a generic method for designing biomaterials for tissue-engineering.
通过表面形貌来调节细胞行为和功能在组织工程中引起了广泛关注。本文制备了一种梯度纤维支架，它由各向异性取向纤维和各向同性退火纤维组成，为细胞迁移、黏附和铺展提供了可控的方向。通过选择溶剂蒸气退火（SVA）诱导的嵌段共聚物（BCP）自组装，对静电纺丝的取向纤维进行刻蚀，形成具有微纳级粗糙度的表面梯度。通过原位/非原位小角 X 射线散射（SAXS）、扫描电子显微镜（SEM）、原子力显微镜（AFM）和体外细胞培养，展示了具有定制自组装纳米结构和焊接纤维微观结构的退火纤维支架对细胞行为的明显调控作用。在蛋白质表达水平上进一步深入研究了集成梯度纤维支架的效果。从梯度拓扑的角度来看，基于 BCP 纤维的这种区域特异性支架显示出指导细胞迁移、黏附和铺展的前景，为组织工程设计生物材料提供了一种通用方法。
