Analytical Services Laboratory, Chemistry and Technology Division, Malaysian Cocoa Board, Cocoa Innovation and Technology Centre, Lot 12621 Kawasan Perindustrian Nilai, 71800 Nilai, Negeri Sembilan, Malaysia.
Food Chem. 2022 Jan 30;368:130778. doi: 10.1016/j.foodchem.2021.130778. Epub 2021 Aug 6.
Multiresidue quantitative and qualitative screening method for the analysis of pesticide residues in dried cocoa beans was validated and applied to imported and domestic cocoa beans samples. The quantitative method comprises of 15 pesticides while the screening method covers 110 pesticides of different chemical classes. The method was based on modified QuEChERS (Quick Easy Cheap Efficient Rugged Safe) extraction and detection using triple quadrupole (QQQ-MS) and ion mobility quadrupole time of flight mass spectrometry (IMS-QTOF). The method was quantitatively validated in terms of linearity, limit of quantification (LOQ), specificity, selectivity, accuracy, and precision. On the other hand, screening detection limits were established for 110 pesticides. Finally, the optimized strategy was successfully applied for the routine analysis of pesticide residues in 137 cocoa bean samples and 32% of the total samples were found positive for ametryn, chlorpyrifos, isoprocarb, and metalaxyl.
多残留定量和定性筛选方法用于分析干燥可可豆中的农药残留，并应用于进口和国产可可豆样品。定量方法包括 15 种农药，而筛选方法涵盖了 110 种不同化学类别的农药。该方法基于改良的 QuEChERS（快速、简单、廉价、有效、坚固、安全）提取，使用三重四极杆（QQQ-MS）和离子淌度四极杆飞行时间质谱（IMS-QTOF）进行检测。该方法在线性、定量限（LOQ）、特异性、选择性、准确性和精密度方面进行了定量验证。另一方面，为 110 种农药建立了筛选检测限。最后，该优化策略成功应用于 137 个可可豆样品中农药残留的常规分析，总样品的 32%检测出莠去津、毒死蜱、异丙威和甲霜灵阳性。
