Department of Plastic Surgery, Peking University Third Hospital, Beijing, China.
Medical College of Soochow University, Suzhou, Jiangsu, China.
J Biochem Mol Toxicol. 2021 Oct;35(10):e22881. doi: 10.1002/jbt.22881. Epub 2021 Aug 15.
Pathological scars and chronic wounds caused by injury, aging, or surgery have always been important public health problems, and there is an urgent need to study the driving forces to find more effective treatments. In this study, we extracted and identified ADSCs exosomes and found that they have the ability to protect HaCat cells from oxidative damage, including promoting proliferation and migration and reducing apoptosis. Further studies determined that the expression of miR-378 was significantly enriched in exosomes. Studies have found that miR-378 mimic can produce protection similar to ADSCs-exo. However, when miR-378 inhibitors are used on ADSCs, the damage protection of the secreted exosomes disappears. This proves that miR-378 enriched in exosomes can improve HaCat's oxidative stress damage. Luciferase experiments show that this effect is achieved by targeting caspase-3. These results indicate that ADSCs play a protective role in wound healing by secreting miR-378-rich exosomes.
病理性瘢痕和由损伤、衰老或手术引起的慢性创面一直是重要的公共健康问题，迫切需要研究其驱动力，以寻找更有效的治疗方法。在这项研究中，我们提取并鉴定了 ADSCs 外泌体，发现它们具有保护 HaCat 细胞免受氧化损伤的能力，包括促进增殖和迁移以及减少细胞凋亡。进一步的研究确定 miR-378 的表达在外泌体中显著富集。研究发现，miR-378 模拟物可以产生类似于 ADSC-exo 的保护作用。然而，当 miR-378 抑制剂用于 ADSCs 时，分泌的外泌体的损伤保护作用消失。这证明了外泌体中富含的 miR-378 可以改善 HaCat 的氧化应激损伤。荧光素酶实验表明，这种作用是通过靶向 caspase-3 实现的。这些结果表明，ADSCs 通过分泌富含 miR-378 的外泌体在伤口愈合中发挥保护作用。
