Yang Xiaoyun, Zhang Shasha, Chen Kewei, Shen Dongsheng, Yang Yang, Shen Aiqun, Liang Junhua, Xu Mengjiao, Yang Yuanyuan, Zhao Yanhong, Li Huaifang, Tong Xiaowen
Department of Obstetrics and Gynecology, Tongji Hospital, Tongji University School of Medicine, No. 389 Xincun Road, Shanghai, 200065 People's Republic of China.
Department of Anesthesiology, Tongji Hospital, Tongji University School of Medicine, No. 389 Xincun Road, Shanghai, 200065 People's Republic of China.
Cell Mol Bioeng. 2024 Sep 4;17(4):295-303. doi: 10.1007/s12195-024-00814-1. eCollection 2024 Aug.
Accelerating wound healing is a main consideration in surgery. The three stages of wound healing are inflammatory response, tissue repair and cell proliferation. Much research has focused on epidermal cell proliferation and migration because this is an essential step in wound healing.
The current study discovered that exosomes from Adipose-derived stem cell (ADSC) following hypoxic preconditioning (HExo) have a greater promotional effect on vaginal wound healing. Protein kinase B (AKT)/hypoxia-inducible factor 1-alpha (HIF-1α) play an important role in HExo-mediated HaCaT cell migration and proliferation. The promotional effect of HExo on rat wound healing was reversed by both, HIF‑1α and AKT inhibition. Phosphorylation of AKT (p-AKT) or HIF‑1α suppression reversed the protective effect of HExo on vaginal wound healing.
Taken together, our study found that hypoxic preconditioning of adipose MSC exosomes enhances vaginal wound healing via accelerated keratinocyte proliferation and migration through AKT/HIF‑1α axis activation.
加速伤口愈合是外科手术中的一个主要考量因素。伤口愈合的三个阶段为炎症反应、组织修复和细胞增殖。许多研究都聚焦于表皮细胞增殖和迁移，因为这是伤口愈合的关键步骤。
当前研究发现，缺氧预处理后的脂肪干细胞来源外泌体（HExo）对阴道伤口愈合具有更强的促进作用。蛋白激酶B（AKT）/缺氧诱导因子1α（HIF-1α）在HExo介导的HaCaT细胞迁移和增殖中发挥重要作用。HIF-1α和AKT抑制均可逆转HExo对大鼠伤口愈合的促进作用。AKT磷酸化（p-AKT）或HIF-1α抑制可逆转HExo对阴道伤口愈合的保护作用。
综上所述，我们的研究发现，脂肪间充质干细胞外泌体的缺氧预处理通过激活AKT/HIF-1α轴加速角质形成细胞增殖和迁移，从而增强阴道伤口愈合。
