A simple method to identify the vesicular and prostatic origin of human seminal components.

Studies on the distribution patterns of certain components in split ejaculates provide information on the origin of seminal constituents in the reproductive tract. In this regard, a simple fractionation method of whole ejaculates has been developed. Human ejaculates after collecting as a whole, were almost equally divided into five fractions through liquefaction known vesicular, prostatic and testicular components were measured in these subsequently liquefied fractions. Levels of vesicular components increased while that of prostatic components decreased gradually and significantly in the subsequent fractions. This change pattern agrees well with that of the fractions in split ejaculates. But the change pattern for spermatozoal concentration was statistically invalid. The bases of these observations have been discussed. The results suggest that studies on the distribution patterns of seminal components in the parts separated during gradual liquefaction can give preliminary ideas about their origin in the reproductive tract like split ejaculation technique.