Paper chromatographic estimation of fructose and myo-inositol in human seminal fluid: a method for evaluating seminal vesicle and prostatic function.

A semiquantitative paper chromatographic method for the separation of myo-inositol and fructose in human seminal fluid was described. The intensity of the chromatographic zones characteristic of myo-inositol, which is a marker for human prostatic secretion and for fructose, which is useful in evaluating seminal vesicle function, were estimated after staining with an alkaline silver nitrate reagent. Characteristic patterns were found for patients with prostatic or vesicular dysfunction, and for patients with agenesis or blockage of the vas deferens. Paper chromatographic analysis of split ejaculate samples allowed evaluation of the sequence of ejaculation of the prostate and seminal vesicles.