Fiehn N E
Department of Microbiology, Royal Dental College, Copenhagen, Denmark.
Acta Pathol Microbiol Immunol Scand B. 1987 Dec;95(6):325-9. doi: 10.1111/j.1699-0463.1987.tb03133.x.
The purpose of the study was to examine the susceptibility of two different small-sized spirochete morphotypes from subgingival plaque to eight antibiotics and chlorhexidine. MIC-values were determined by a broth dilution method and related to achievable antibiotic tissue- and blood concentrations. The spirochetes were characterized as susceptible, moderate susceptible, and resistant to the different antibiotics. The MICs of tetracycline hydrochloride, doxycycline, penicillin G, and chlorhexidine were all considerably lower for spirochete strains with a 2:4:2 endoflagella system (two endoflagella from each cell-end) than for strains with a 1:2:1 endoflagella system (one endoflagellum from each cell-end). No differences were observed for the remaining antibiotics. Spirochetes containing one endoflagellum from each cell-end were found to be susceptible to metronidazole and doxycycline, susceptible to moderate susceptible to tetracycline hydrochloride, moderate susceptible to penicillin G, and resistant to the remaining antibiotics. Spirochetes containing two endoflagella from each cell-end were susceptible to doxycycline, tetracycline hydrochloride, and metronidazole. Susceptible to moderate susceptible to penicillin G. These spirochetes were resistant to the remaining agents. The significance of these observations is discussed in relation to obtainable gingival crevicular fluid concentrations and treatment of marginal periodontitis.
本研究的目的是检测来自龈下菌斑的两种不同小型螺旋体形态型对八种抗生素和洗必泰的敏感性。通过肉汤稀释法测定最低抑菌浓度（MIC）值，并将其与可达到的抗生素组织浓度和血液浓度相关联。将螺旋体表征为对不同抗生素敏感、中度敏感和耐药。对于具有2:4:2内鞭毛系统（每个细胞末端有两条内鞭毛）的螺旋体菌株，盐酸四环素、强力霉素、青霉素G和洗必泰的MIC均显著低于具有1:2:1内鞭毛系统（每个细胞末端有一条内鞭毛）的菌株。其余抗生素未观察到差异。发现每个细胞末端含有一条内鞭毛的螺旋体对甲硝唑和强力霉素敏感，对盐酸四环素敏感至中度敏感，对青霉素G中度敏感，对其余抗生素耐药。每个细胞末端含有两条内鞭毛的螺旋体对强力霉素、盐酸四环素和甲硝唑敏感。对青霉素G敏感至中度敏感。这些螺旋体对其余药物耐药。结合可获得的龈沟液浓度和边缘性牙周炎的治疗讨论了这些观察结果的意义。
