Fiehn N E
Department of Microbiology, Royal Dental College, Copenhagen, Denmark.
Acta Pathol Microbiol Immunol Scand B. 1987 Oct;95(5):309-14. doi: 10.1111/j.1699-0463.1987.tb03130.x.
The purpose of the present investigation was to establish a biochemical characterization of oral spirochetes containing one endoflagellum from each cell-end. Nine spirochete strains were isolated from subgingival plaque with pocket depth greater than 6 mm. The following metabolic capabilities were examined: fermentation of 16 different carbohydrates, hydrolysis of urea, gelatin and esculin, and production of indol and H2S. Furthermore activities of the following categories of enzymes were examined: proteases, peptidases, lipases, glycosidases, phosphatases, and mucopolysaccharadases. The tests and analyses were routinely carried out with cultures in the early stationary phase of growth. Of the examined metabolic capabilities eleven of the 21 characters were identical for all strains. Only the fermentation of some of the carbohydrates varied between the strains. All strains were identical regarding the examined enzymes. The following enzyme activities were found: acid and alkaline phosphatase, C-4 (butyrate)-, and C-8 (caprylate)-lipases, peptidases, hyaluronidase, and chondroitinsulfatase. The findings are compared with earlier observations for the same spirochete morphotype and with small-sized spirochetes containing two endoflagella from each cell-end.
本研究的目的是对每端含一条内鞭毛的口腔螺旋体进行生化特性鉴定。从牙周袋深度大于6mm的龈下菌斑中分离出9株螺旋体菌株。检测了以下代谢能力：16种不同碳水化合物的发酵、尿素、明胶和七叶苷的水解以及吲哚和H2S的产生。此外，还检测了以下几类酶的活性：蛋白酶、肽酶、脂肪酶、糖苷酶、磷酸酶和粘多糖酶。测试和分析通常在生长的早期稳定期培养物中进行。在所检测的代谢能力中，21个特征中有11个对所有菌株来说是相同的。只有某些碳水化合物的发酵在菌株之间有所不同。所有菌株在所检测的酶方面是相同的。发现了以下酶活性：酸性和碱性磷酸酶、C-4（丁酸）-和C-8（辛酸）-脂肪酶、肽酶、透明质酸酶和硫酸软骨素酶。将这些发现与之前对相同螺旋体形态型以及每端含两条内鞭毛的小型螺旋体的观察结果进行了比较。
