Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Ruhuna, Galle, Sri Lanka.
Department of Biochemistry, Faculty of Medicine, University of Ruhuna, Galle, Sri Lanka.
J Food Biochem. 2021 Sep;45(9):e13901. doi: 10.1111/jfbc.13901. Epub 2021 Aug 16.
The nephroprotective effect of standardized aqueous root extract of Vetiveria zizanioides (L.) Nash (Family: Poaceae) was investigated in doxorubicin-induced (20 mg/kg, ip) experimental nephrotoxicity model of Wistar rats. The freeze-dried aqueous refluxed (4 hr) root extract of V. zizanioides (25, 50; equivalent human therapeutic dose and 100 mg/kg) was administered separately to nephrotoxic Wistar rats (n = 6/group). Supplement of V. zizanioides resulted a dose-dependent reduction in raised serum creatinine, β -microglobulin, and blood urea nitrogen and a subsequent increase in serum total protein and albumin in nephrotoxic rats (p < .05). An attenuation of the doxorubicin-induced features of renal parenchymal injury was observed on H- and E-stained sections of the kidney tissues. Nootkatone, dehydroaromadendrene, isokhusenic acid, α-vetivone, and isolongifolene were identified in the methanol extract of V. zizanioides based on the GC-MS chromatogram analysis. The findings revealed that the supplement of standardized aqueous root extract of V. zizanioides had a significant dose-dependent nephroprotective activity against doxorubicin-induced experimental nephrotoxicity. PRACTICAL APPLICATIONS: Vetiveria zizanioides is a medicinal plant with a variety of therapeutic applications in kidney-related diseases. Apparently, it is used as a food ingredient due to its fresh and elegant scent and potential bioactivities. The aqueous root extract of V. zizanioides exerted relatively high antioxidant potential in vitro, substantiating the health effects of the plant pertaining to kidney diseases as a potential source of dietary antioxidant. The administration of the plant extract resulted in significant nephroprotection against doxorubicin-induced experimental nephrotoxicity revealing the significance of V. zizanioides as a promising dietary supplement in the management of kidney disease.
对香根草（禾本科）标准化水根提取物的肾保护作用进行了研究，该提取物来自多柔比星（20mg/kg，ip）诱导的 Wistar 大鼠实验性肾病模型。将香根草的冻干水回流（4 小时）根提取物（25、50；相当于人体治疗剂量和 100mg/kg）分别施用于肾毒性 Wistar 大鼠（每组 6 只）。香根草补充剂可使升高的血清肌酐、β-微球蛋白和血尿素氮呈剂量依赖性降低，并使肾毒性大鼠的血清总蛋白和白蛋白随后增加（p<0.05）。在肾组织 H-E 染色切片中观察到对多柔比星诱导的肾实质损伤特征的减弱。根据 GC-MS 色谱图分析，在香根草甲醇提取物中鉴定出诺卡酮、脱氢芳樟醇、异胡薄荷酮、α-香根草酮和异长叶烯。研究结果表明，标准化水根提取物的补充对多柔比星诱导的实验性肾病毒性具有显著的剂量依赖性肾保护活性。实际应用：香根草是一种药用植物，在肾脏相关疾病方面具有多种治疗应用。显然，由于其清新优雅的香气和潜在的生物活性，它被用作食品成分。香根草的水根提取物在体外具有相对较高的抗氧化潜力，这证实了该植物对肾脏疾病的健康影响，因为它是膳食抗氧化剂的潜在来源。植物提取物的给药对多柔比星诱导的实验性肾病毒性具有显著的肾保护作用，这表明香根草作为一种有前途的膳食补充剂，在肾脏疾病的治疗中有重要意义。
