Luqman Suaib, Kumar Ritesh, Kaushik Shubhangi, Srivastava Suchita, Darokar Mahendra P, Khanuja Suman P S
Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (Council of Scientific and Industrial Research), P.O. CIMAP, Lucknow 226 015, India
Indian J Biochem Biophys. 2009 Feb;46(1):122-5.
Vetiveria zizanioides, an aromatic plant commonly known as vetiver has been used for various ailments. The essential oil of vetiver root has been shown to possess antioxidant activity. However, antioxidant potential of spent root extract has not been reported. Hence, in the present study, ferric reducing, free radical scavenging and antioxidant activity of two genotypes namely KS1 and gulabi of V. zizanioides L. Nash root were investigated using in vitro assays - the ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), total phenolic content (TPC), total antioxidant capacity (TAC) and reducing power (RP). KS1 genotype showed higher FRAP values, DPPH inhibition, TPC and RP potential compared to gulabi and the antioxidant activity increased with the concentration of the extract (10-1000 microg/mL). A significant protective effect of cv KS1 (100 microg/mL) extract was also observed in reduced glutathione (GSH) and malondialdehyde (MDA) concentrations of erythrocytes subjected to oxidative stress by tert-butyl hydroperoxide (t-BHP) and hydrogen peroxide (H202). The cv KS1 showed better antioxidant activity, compared to cv gulabi indicating the possibility of exploring the presence of different phytoconstituents in the two varieties.
香根草，一种通常被称为香根的芳香植物，已被用于治疗各种疾病。香根草根部的精油已被证明具有抗氧化活性。然而，废弃根提取物的抗氧化潜力尚未见报道。因此，在本研究中，使用体外测定法——铁还原抗氧化能力（FRAP）、1,1-二苯基-2-苦基肼（DPPH）、总酚含量（TPC）、总抗氧化能力（TAC）和还原能力（RP），对香根草L. Nash根的两个基因型KS1和古拉比的铁还原、自由基清除和抗氧化活性进行了研究。与古拉比相比，KS1基因型表现出更高的FRAP值、DPPH抑制率、TPC和RP潜力，并且抗氧化活性随提取物浓度（10 - 1000微克/毫升）的增加而增强。在经叔丁基过氧化氢（t - BHP）和过氧化氢（H2O2）诱导氧化应激的红细胞中，还观察到cv KS1（100微克/毫升）提取物对谷胱甘肽（GSH）和丙二醛（MDA）浓度具有显著的保护作用。与cv古拉比相比，cv KS1表现出更好的抗氧化活性，这表明有可能探索这两个品种中不同植物成分的存在情况。
