Cai Yun-Shuang, Li Yong, Chen Zhao-Chan, Yu Shi-Shan
Guangxi University of Chinese Medicine Nanning 530200,China.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100050,China.
Zhongguo Zhong Yao Za Zhi. 2021 Jul;46(13):3368-3376. doi: 10.19540/j.cnki.cjcmm.20210331.201.
This study aims to investigate Erythrina alkaloids from the stems of Erythrina corallodendron. Eighteen Erythrina alkaloids were isolated from the 95% ethanol extract of the stems of E. corallodendron by silica gel,octadecyl silica( ODS),Sephadex LH-20 column chromatography and preparative HPLC. With nuclear magnetic resonance( NMR) spectroscopy and mass spectrometry( MS),their structures were identified as crstanine A( 1),erytharbine( 2),cristamine C( 3),( +)-erystramindine( 4),10,11-dioxoerythraline( 5),8-oxoerythraline( 6),8-oxo-11β-methoxyerythradine( 7),11-methoxyerythradine( 8),( ±)-11-epi-methoxyerythraline( 9),( +)-erythraline( 10),crystamidine( 11),8-oxoerythrinine( 12),( +)-11α-hydroxyerysotrine( 13),erythrinine( 14),erysodine( 15),erysotrine-N-oxide( 16),( +)-erythratidine( 17),erythratine( 18). Compounds 1-4,7,9,11,13,16 and 17 were isolated from E. corallodendron for the first time. Furthermore,the cytotoxic activities of these Erythrina alkaloids were screened by MTT assay. The results showed that all compounds had no obvious cytotoxic activity. The analgesic activities of compounds1,6 and 8 were evaluated using an acetic acid-induced writhing test in mice. The writhing inhibition rates of compounds 1,6 and 8 at20 mg·kg~(-1)( ip) were 69%,70% and 62%,respectively( P<0. 01),indicating they have significant analgesic activity.
本研究旨在对珊瑚刺桐茎中的刺桐生物碱进行研究。通过硅胶柱色谱、十八烷基硅胶（ODS）柱色谱、葡聚糖凝胶LH - 20柱色谱和制备型高效液相色谱，从珊瑚刺桐茎的95%乙醇提取物中分离得到了18种刺桐生物碱。利用核磁共振（NMR）光谱和质谱（MS）对其结构进行鉴定，它们分别为刺桐宁A（1）、刺桐宾（2）、刺桐胺C（3）、（+）-刺桐明定（4）、10,11 - 二氧刺桐灵（5）、8 - 氧代刺桐灵（6）、8 - 氧代 - 11β - 甲氧基刺桐定（7）、11 - 甲氧基刺桐定（8）、（±）-11 - 表甲氧基刺桐灵（9）、（+）-刺桐灵（10）、刺桐脒（11）、8 - 氧代刺桐宁（12）、（+）-11α - 羟基刺桐碱（13）、刺桐宁（14）、刺桐豆碱（15）、刺桐碱 - N - 氧化物（16）、（+）-刺桐替定（17）、刺桐替宁（18）。化合物1 - 4、7、9、11、13、16和17首次从珊瑚刺桐中分离得到。此外，采用MTT法对这些刺桐生物碱的细胞毒活性进行了筛选。结果表明，所有化合物均无明显的细胞毒活性。采用小鼠醋酸扭体试验对化合物1、6和8的镇痛活性进行了评价。化合物1、6和8在20 mg·kg⁻¹（腹腔注射）时的扭体抑制率分别为69%、70%和62%（P < 0.01），表明它们具有显著的镇痛活性。
