Wang Zhen-Yu, Yang Si-Yue, Lyu Wei, Ding Jian-Bao, Ma Jian-Long, Yang Jin
School of Chemistry and Chemical Engineering,North Minzu University Yinchuan 750021,China Key Laboratory of Chemical Engineering and Technology,State Ethnic Affairs Commission Yinchuan 750021,China.
Department of Statistical Sciences,University of Toronto Toronto M5T 1P5,Canada.
Zhongguo Zhong Yao Za Zhi. 2021 Jul;46(13):3377-3387. doi: 10.19540/j.cnki.cjcmm.20210308.201.
The chemical components of Lycii Fructus were analyzed by liquid chromatography( LC) and mass spectrometry( MS for the establishment of spectrum-activity relationship,on the basis of which its antioxidant active ingredients were determined. In this experiment,Lycii Fructus was extracted with different solvents and then separated into 80 samples by macroporous adsorption resin and reversed-phase chromatography,respectively. The antioxidant components were enriched into 11 samples and their scavenging abilities against DPPH free radical and ferric ion reducing antioxidant power( FRAP) were significantly stronger than those before the treatment( P<0. 05). The spectrum-activity relationship regarding the antioxidant activity in vitro of Lycii Fructus was established by Pearson correlation analysis,orthogonal partial least squares( OPLS) and elastic net regression. Six chromatographic peaks greatly contributing to the antioxidant activity in vitro of Lycii Fructus were identified as rutin( P6),quercetin( P35),scopoletin( P14),N-cis-feruloyl-4-O-β-D-glucopyranosyl-tyramine or N-( 4-O-β-D-glucopyranosyl-trans-feruloyl)-tyramine( P8), ferulic acid( P13) and1,3,5-dihydroxy-2-isoprenyl-3-xanthone( P23). The active components associated with free radical scavenging were rutin and quercetin both belonging to flavonoids. The reduction of Fe3+was based on phenylpropanoids such as ferulic acid,scopoletin,xanthone and phenolic amides. These results indicated that the antioxidant activity of Lycii Fructus was ascribed to the synergistic action of different products through different ways. Besides,the data analysis model should be chosen carefully for the establishment of spectrum-activity relationship,thus ensuring the reliability of results.
采用液相色谱（LC）和质谱（MS）对枸杞子的化学成分进行分析，以建立谱效关系，并在此基础上确定其抗氧化活性成分。本实验中，用不同溶剂对枸杞子进行提取，然后分别通过大孔吸附树脂和反相色谱法将其分离为80个样品。将抗氧化成分富集到11个样品中，其对DPPH自由基的清除能力和铁离子还原抗氧化能力（FRAP）均显著强于处理前（P<0.05）。通过Pearson相关分析、正交偏最小二乘法（OPLS）和弹性网络回归建立了枸杞子体外抗氧化活性的谱效关系。确定了对枸杞子体外抗氧化活性有很大贡献的6个色谱峰，分别为芦丁（P6）、槲皮素（P35）、东莨菪素（P14）、N-顺式阿魏酰基-4-O-β-D-吡喃葡萄糖基酪胺或N-（4-O-β-D-吡喃葡萄糖基-反式阿魏酰基）-酪胺（P8）、阿魏酸（P13）和1,3,5-三羟基-2-异戊烯基-3-呫吨酮（P23）。与自由基清除相关的活性成分是芦丁和槲皮素，均属于黄酮类化合物。Fe3+的还原基于阿魏酸、东莨菪素、呫吨酮和酚酰胺等苯丙素类化合物。这些结果表明，枸杞子的抗氧化活性归因于不同产物通过不同方式的协同作用。此外，在建立谱效关系时应谨慎选择数据分析模型，从而确保结果的可靠性。
